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COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting
As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has surged across the globe, great effort has been expended to understand mechanisms of transmission and spread. From a hospital perspective, this topic is critical to limit and prevent SARS-CoV-2 iatrogenic transmission within the heal...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461048/ https://www.ncbi.nlm.nih.gov/pubmed/34219476 http://dx.doi.org/10.1177/15353702211024597 |
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author | Bartlett, Christopher Langsjoen, Jens Cheng, Qiuying Yingling, Alexandra V Weiss, Myissa Bradfute, Steven Perkins, Douglas J Hurwitz, Ivy |
author_facet | Bartlett, Christopher Langsjoen, Jens Cheng, Qiuying Yingling, Alexandra V Weiss, Myissa Bradfute, Steven Perkins, Douglas J Hurwitz, Ivy |
author_sort | Bartlett, Christopher |
collection | PubMed |
description | As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has surged across the globe, great effort has been expended to understand mechanisms of transmission and spread. From a hospital perspective, this topic is critical to limit and prevent SARS-CoV-2 iatrogenic transmission within the healthcare environment. Currently, the virus is believed to be transmitted primarily through respiratory droplets, but a growing body of evidence suggests that spread is also possible through aerosolized particles and fomites. Amidst a growing volume of patients with coronavirus disease 2019 (COVID-19), the purpose of this study was to evaluate the potential for SARS-CoV-2 transmission through fomites. Samples collected from the exposed skin of clinicians (n = 42) and high-touch surfaces (n = 40) were collected before and after encounters with COVID-19 patients. Samples were analyzed using two assays: the CDC 2019-nCoV Real-Time Reverse Transcription polymerase chain reaction (RT-qPCR) assay, and a SYBR Green assay that targeted a 121 bp region within the S-gene of SARS-CoV-2. None of the samples tested positive with the CDC assay, while two high-touch surface areas tested positive for SARS-CoV-2 using the Spike assay. However, viral culture did not reveal viable SARS-CoV-2 from the positive samples. Overall, the results from this study suggest that SARS-CoV-2 RNA were not widely present either on exposed skin flora or high-touch surface areas in the hospital locations tested. The inability to recover viable virus from samples that tested positive by the molecular assays, however, does not rule out the possibility of SARS-CoV-2 transmission through fomites. |
format | Online Article Text |
id | pubmed-8461048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-84610482021-09-25 COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting Bartlett, Christopher Langsjoen, Jens Cheng, Qiuying Yingling, Alexandra V Weiss, Myissa Bradfute, Steven Perkins, Douglas J Hurwitz, Ivy Exp Biol Med (Maywood) Original Research As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has surged across the globe, great effort has been expended to understand mechanisms of transmission and spread. From a hospital perspective, this topic is critical to limit and prevent SARS-CoV-2 iatrogenic transmission within the healthcare environment. Currently, the virus is believed to be transmitted primarily through respiratory droplets, but a growing body of evidence suggests that spread is also possible through aerosolized particles and fomites. Amidst a growing volume of patients with coronavirus disease 2019 (COVID-19), the purpose of this study was to evaluate the potential for SARS-CoV-2 transmission through fomites. Samples collected from the exposed skin of clinicians (n = 42) and high-touch surfaces (n = 40) were collected before and after encounters with COVID-19 patients. Samples were analyzed using two assays: the CDC 2019-nCoV Real-Time Reverse Transcription polymerase chain reaction (RT-qPCR) assay, and a SYBR Green assay that targeted a 121 bp region within the S-gene of SARS-CoV-2. None of the samples tested positive with the CDC assay, while two high-touch surface areas tested positive for SARS-CoV-2 using the Spike assay. However, viral culture did not reveal viable SARS-CoV-2 from the positive samples. Overall, the results from this study suggest that SARS-CoV-2 RNA were not widely present either on exposed skin flora or high-touch surface areas in the hospital locations tested. The inability to recover viable virus from samples that tested positive by the molecular assays, however, does not rule out the possibility of SARS-CoV-2 transmission through fomites. SAGE Publications 2021-07-04 2021-09 /pmc/articles/PMC8461048/ /pubmed/34219476 http://dx.doi.org/10.1177/15353702211024597 Text en © 2021 by the Society for Experimental Biology and Medicine https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Bartlett, Christopher Langsjoen, Jens Cheng, Qiuying Yingling, Alexandra V Weiss, Myissa Bradfute, Steven Perkins, Douglas J Hurwitz, Ivy COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in a university hospital setting |
title | COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in
a university hospital setting |
title_full | COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in
a university hospital setting |
title_fullStr | COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in
a university hospital setting |
title_full_unstemmed | COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in
a university hospital setting |
title_short | COVID-19 global pandemic planning: Presence of SARS-CoV-2 fomites in
a university hospital setting |
title_sort | covid-19 global pandemic planning: presence of sars-cov-2 fomites in
a university hospital setting |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461048/ https://www.ncbi.nlm.nih.gov/pubmed/34219476 http://dx.doi.org/10.1177/15353702211024597 |
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