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Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP

A 4-nitrophenol-degrading bacterial strain PNP was isolated from pesticide-contaminated soil collected from Lucknow. Strain PNP utilized 0.5 mM 4-nitrophenol as its carbon source and degraded it completely within 24 h with stoichiometric release of nitrite ions. Strain PNP was associated with the ge...

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Autores principales: Arora, Pankaj Kumar, Saroj, Raj Shekhar, Mishra, Rupali, Omar, Rishabh Anand, Kumari, Puja, Srivastava, Alok, Garg, Sanjay Kumar, Singh, Vijay Pal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461342/
https://www.ncbi.nlm.nih.gov/pubmed/34589566
http://dx.doi.org/10.1016/j.dib.2021.107390
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author Arora, Pankaj Kumar
Saroj, Raj Shekhar
Mishra, Rupali
Omar, Rishabh Anand
Kumari, Puja
Srivastava, Alok
Garg, Sanjay Kumar
Singh, Vijay Pal
author_facet Arora, Pankaj Kumar
Saroj, Raj Shekhar
Mishra, Rupali
Omar, Rishabh Anand
Kumari, Puja
Srivastava, Alok
Garg, Sanjay Kumar
Singh, Vijay Pal
author_sort Arora, Pankaj Kumar
collection PubMed
description A 4-nitrophenol-degrading bacterial strain PNP was isolated from pesticide-contaminated soil collected from Lucknow. Strain PNP utilized 0.5 mM 4-nitrophenol as its carbon source and degraded it completely within 24 h with stoichiometric release of nitrite ions. Strain PNP was associated with the genus Pseudomonas in a phylogentic tree and exhibited highest 16S rRNA gene sequence similarity to Pseudomonas juntendi BML3 (99.79%) and Pseudomonas inefficax JV551A3 (99.79%). Based on values of average nucleotide identity and digital DNA-DNA hybridization among strain PNP and its closely related type strains, it concluded that strain PNP belongs to Pseudomonas alloputida. The Illumina HiSeq platform was used to sequence the PNP genome. The draft genome sequence of Pseudomonas alloputida PNP was presented here. The total size of the draft assembly was 6,087,340 bp, distributed into 87 contigs with N50 value of 139502. The genome has an average GC content of 61.7% and contains 5461 coding sequences and 77 putative RNA genes. This Whole Genome Shotgun project has been submitted at DDBJ/ENA/GenBank under the accession JAGKJH000000000.
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spelling pubmed-84613422021-09-28 Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP Arora, Pankaj Kumar Saroj, Raj Shekhar Mishra, Rupali Omar, Rishabh Anand Kumari, Puja Srivastava, Alok Garg, Sanjay Kumar Singh, Vijay Pal Data Brief Data Article A 4-nitrophenol-degrading bacterial strain PNP was isolated from pesticide-contaminated soil collected from Lucknow. Strain PNP utilized 0.5 mM 4-nitrophenol as its carbon source and degraded it completely within 24 h with stoichiometric release of nitrite ions. Strain PNP was associated with the genus Pseudomonas in a phylogentic tree and exhibited highest 16S rRNA gene sequence similarity to Pseudomonas juntendi BML3 (99.79%) and Pseudomonas inefficax JV551A3 (99.79%). Based on values of average nucleotide identity and digital DNA-DNA hybridization among strain PNP and its closely related type strains, it concluded that strain PNP belongs to Pseudomonas alloputida. The Illumina HiSeq platform was used to sequence the PNP genome. The draft genome sequence of Pseudomonas alloputida PNP was presented here. The total size of the draft assembly was 6,087,340 bp, distributed into 87 contigs with N50 value of 139502. The genome has an average GC content of 61.7% and contains 5461 coding sequences and 77 putative RNA genes. This Whole Genome Shotgun project has been submitted at DDBJ/ENA/GenBank under the accession JAGKJH000000000. Elsevier 2021-09-20 /pmc/articles/PMC8461342/ /pubmed/34589566 http://dx.doi.org/10.1016/j.dib.2021.107390 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Arora, Pankaj Kumar
Saroj, Raj Shekhar
Mishra, Rupali
Omar, Rishabh Anand
Kumari, Puja
Srivastava, Alok
Garg, Sanjay Kumar
Singh, Vijay Pal
Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP
title Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP
title_full Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP
title_fullStr Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP
title_full_unstemmed Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP
title_short Draft genome sequence data of a 4-nitrophenol- degrading bacterium, Pseudomonas alloputida strain PNP
title_sort draft genome sequence data of a 4-nitrophenol- degrading bacterium, pseudomonas alloputida strain pnp
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461342/
https://www.ncbi.nlm.nih.gov/pubmed/34589566
http://dx.doi.org/10.1016/j.dib.2021.107390
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