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Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study

OBJECTIVE: E. faecalis is one of the most commonly found species in persistent and secondary infections associated with endodontic failure. This in vitro study aimed to evaluate the depth of penetration and antimicrobial efficacy of 5% and 10% bamboo salt (BS), 2% chlorhexidine gel (CHX) and calcium...

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Autores principales: Kumar, Palmoor Santosh, Vidhya, Sampath, Mahalaxmi, Sekar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kare Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461498/
https://www.ncbi.nlm.nih.gov/pubmed/34650016
http://dx.doi.org/10.14744/eej.2021.09709
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author Kumar, Palmoor Santosh
Vidhya, Sampath
Mahalaxmi, Sekar
author_facet Kumar, Palmoor Santosh
Vidhya, Sampath
Mahalaxmi, Sekar
author_sort Kumar, Palmoor Santosh
collection PubMed
description OBJECTIVE: E. faecalis is one of the most commonly found species in persistent and secondary infections associated with endodontic failure. This in vitro study aimed to evaluate the depth of penetration and antimicrobial efficacy of 5% and 10% bamboo salt (BS), 2% chlorhexidine gel (CHX) and calcium hydroxide (CH) against E. faecalis. METHODS: E. faecalis was inoculated in dentine blocks for 21 days following which the antibacterial efficacy of the experimental medicaments were quantatively assessed by harvesting the dentinal debris from depths of 200 µm and 400 µm from the block lumen on the 2(nd) and 7(th) day. The depth of penetration of the medicaments was measured using LIVE/DEAD BacLight stain under CLSM. RESULTS: Results showed that the medicaments had varying degrees of antimicrobial efficacy and depth of penetration. Among the medicaments, CHX showed the highest antimicrobial activity on both the time intervals (P<0.05), followed by 10% BS, 5% BS and the least efficacy was observed in CH group. CHX and 10% BS exhibited the highest depth of penetration, which was proximate to the penetration of E. faecalis. CONCLUSION: It can be concluded that 10% bamboo salt can serve as a viable natural antimicrobial in endodontic therapy.
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spelling pubmed-84614982021-09-28 Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study Kumar, Palmoor Santosh Vidhya, Sampath Mahalaxmi, Sekar Eur Endod J Original Article OBJECTIVE: E. faecalis is one of the most commonly found species in persistent and secondary infections associated with endodontic failure. This in vitro study aimed to evaluate the depth of penetration and antimicrobial efficacy of 5% and 10% bamboo salt (BS), 2% chlorhexidine gel (CHX) and calcium hydroxide (CH) against E. faecalis. METHODS: E. faecalis was inoculated in dentine blocks for 21 days following which the antibacterial efficacy of the experimental medicaments were quantatively assessed by harvesting the dentinal debris from depths of 200 µm and 400 µm from the block lumen on the 2(nd) and 7(th) day. The depth of penetration of the medicaments was measured using LIVE/DEAD BacLight stain under CLSM. RESULTS: Results showed that the medicaments had varying degrees of antimicrobial efficacy and depth of penetration. Among the medicaments, CHX showed the highest antimicrobial activity on both the time intervals (P<0.05), followed by 10% BS, 5% BS and the least efficacy was observed in CH group. CHX and 10% BS exhibited the highest depth of penetration, which was proximate to the penetration of E. faecalis. CONCLUSION: It can be concluded that 10% bamboo salt can serve as a viable natural antimicrobial in endodontic therapy. Kare Publishing 2021-08-13 /pmc/articles/PMC8461498/ /pubmed/34650016 http://dx.doi.org/10.14744/eej.2021.09709 Text en Copyright: © 2021 European Endodontic Journal https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Original Article
Kumar, Palmoor Santosh
Vidhya, Sampath
Mahalaxmi, Sekar
Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study
title Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study
title_full Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study
title_fullStr Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study
title_full_unstemmed Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study
title_short Depth of Penetration and Antimicrobial Activity of 5% and 10% Bamboo Salt, 2% Chlorhexidine Gel and Calcium Hydroxide Against Enterococcus faecalis – An In Vitro Study
title_sort depth of penetration and antimicrobial activity of 5% and 10% bamboo salt, 2% chlorhexidine gel and calcium hydroxide against enterococcus faecalis – an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461498/
https://www.ncbi.nlm.nih.gov/pubmed/34650016
http://dx.doi.org/10.14744/eej.2021.09709
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