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Genetic Code Expansion in the Engineered Organism Vmax X2: High Yield and Exceptional Fidelity
[Image: see text] We report that the recently introduced commercial strain of Vibrio natriegens (Vmax X2) supports robust unnatural amino acid mutagenesis, generating exceptional yields of soluble protein containing up to 5 noncanonical α-amino acids (ncAA). The isolated yields of ncAA-containing su...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461772/ https://www.ncbi.nlm.nih.gov/pubmed/34584951 http://dx.doi.org/10.1021/acscentsci.1c00499 |