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Control of vein-forming, striped gene expression by auxin signaling

BACKGROUND: Activation of gene expression in striped domains is a key building block of biological patterning, from the recursive formation of veins in plant leaves to that of ribs and vertebrae in our bodies. In animals, gene expression is activated in striped domains by the differential affinity o...

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Autores principales: Krishna, Anmol, Gardiner, Jason, Donner, Tyler J., Scarpella, Enrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461865/
https://www.ncbi.nlm.nih.gov/pubmed/34556094
http://dx.doi.org/10.1186/s12915-021-01143-9
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author Krishna, Anmol
Gardiner, Jason
Donner, Tyler J.
Scarpella, Enrico
author_facet Krishna, Anmol
Gardiner, Jason
Donner, Tyler J.
Scarpella, Enrico
author_sort Krishna, Anmol
collection PubMed
description BACKGROUND: Activation of gene expression in striped domains is a key building block of biological patterning, from the recursive formation of veins in plant leaves to that of ribs and vertebrae in our bodies. In animals, gene expression is activated in striped domains by the differential affinity of broadly expressed transcription factors for their target genes and the combinatorial interaction between such target genes. In plants, how gene expression is activated in striped domains is instead unknown. We address this question for the broadly expressed MONOPTEROS (MP) transcription factor and its target gene ARABIDOPSIS THALIANA HOMEOBOX FACTOR8 (ATHB8). RESULTS: We find that ATHB8 promotes vein formation and that such vein-forming function depends on both levels of ATHB8 expression and width of ATHB8 expression domains. We further find that ATHB8 expression is activated in striped domains by a combination of (1) activation of ATHB8 expression through binding of peak levels of MP to a low-affinity MP-binding site in the ATHB8 promoter and (2) repression of ATHB8 expression by MP target genes of the AUXIN/INDOLE-3-ACETIC-ACID-INDUCIBLE family. CONCLUSIONS: Our findings suggest that a common regulatory logic controls activation of gene expression in striped domains in both plants and animals despite the independent evolution of their multicellularity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-021-01143-9.
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spelling pubmed-84618652021-09-24 Control of vein-forming, striped gene expression by auxin signaling Krishna, Anmol Gardiner, Jason Donner, Tyler J. Scarpella, Enrico BMC Biol Research Article BACKGROUND: Activation of gene expression in striped domains is a key building block of biological patterning, from the recursive formation of veins in plant leaves to that of ribs and vertebrae in our bodies. In animals, gene expression is activated in striped domains by the differential affinity of broadly expressed transcription factors for their target genes and the combinatorial interaction between such target genes. In plants, how gene expression is activated in striped domains is instead unknown. We address this question for the broadly expressed MONOPTEROS (MP) transcription factor and its target gene ARABIDOPSIS THALIANA HOMEOBOX FACTOR8 (ATHB8). RESULTS: We find that ATHB8 promotes vein formation and that such vein-forming function depends on both levels of ATHB8 expression and width of ATHB8 expression domains. We further find that ATHB8 expression is activated in striped domains by a combination of (1) activation of ATHB8 expression through binding of peak levels of MP to a low-affinity MP-binding site in the ATHB8 promoter and (2) repression of ATHB8 expression by MP target genes of the AUXIN/INDOLE-3-ACETIC-ACID-INDUCIBLE family. CONCLUSIONS: Our findings suggest that a common regulatory logic controls activation of gene expression in striped domains in both plants and animals despite the independent evolution of their multicellularity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-021-01143-9. BioMed Central 2021-09-24 /pmc/articles/PMC8461865/ /pubmed/34556094 http://dx.doi.org/10.1186/s12915-021-01143-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Krishna, Anmol
Gardiner, Jason
Donner, Tyler J.
Scarpella, Enrico
Control of vein-forming, striped gene expression by auxin signaling
title Control of vein-forming, striped gene expression by auxin signaling
title_full Control of vein-forming, striped gene expression by auxin signaling
title_fullStr Control of vein-forming, striped gene expression by auxin signaling
title_full_unstemmed Control of vein-forming, striped gene expression by auxin signaling
title_short Control of vein-forming, striped gene expression by auxin signaling
title_sort control of vein-forming, striped gene expression by auxin signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461865/
https://www.ncbi.nlm.nih.gov/pubmed/34556094
http://dx.doi.org/10.1186/s12915-021-01143-9
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