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Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature

Bacterial infection presents severe challenge to tilapia farming, which is largely influenced by water temperature. However, how water temperature determines tilapias’ survival to infection is not well understood. Here, we address this issue from the perspective of metabolic state. Tilapias were mor...

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Autores principales: Yang, Man-jun, Jiang, Ming, Peng, Xuan-xian, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8462736/
https://www.ncbi.nlm.nih.gov/pubmed/34566956
http://dx.doi.org/10.3389/fimmu.2021.682724
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author Yang, Man-jun
Jiang, Ming
Peng, Xuan-xian
Li, Hui
author_facet Yang, Man-jun
Jiang, Ming
Peng, Xuan-xian
Li, Hui
author_sort Yang, Man-jun
collection PubMed
description Bacterial infection presents severe challenge to tilapia farming, which is largely influenced by water temperature. However, how water temperature determines tilapias’ survival to infection is not well understood. Here, we address this issue from the perspective of metabolic state. Tilapias were more susceptible to Aeromonas sobria infection at 33°C than at 18°C, which is associated with differential metabolism of the fish. Compared to the metabolome of tilapia at 18°C, the metabolome at 33°C was characterized with increased an tricarboxylic acid cycle and a reduced level of myo-inositol which represent the most impactful pathway and crucial biomarker, respectively. These alterations were accompanied with the elevated transcriptional level of 10 innate immune genes with infection time, where il-1b, il-6, il-8, and il-10 exhibited a higher expression at 33°C than at 18°C and was attenuated by exogenous myo-inositol in both groups. Interestingly, exogenous myo-inositol inactivated the elevated TCA cycle via inhibiting the enzymatic activity of succinate dehydrogenase and malate dehydrogenase. Thus, tilapias showed a higher survival ability at 33°C. Our study reveals a previously unknown relationship among water temperature, metabolic state, and innate immunity and establishes a novel approach to eliminate bacterial pathogens in tilapia at higher water temperature.
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spelling pubmed-84627362021-09-25 Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature Yang, Man-jun Jiang, Ming Peng, Xuan-xian Li, Hui Front Immunol Immunology Bacterial infection presents severe challenge to tilapia farming, which is largely influenced by water temperature. However, how water temperature determines tilapias’ survival to infection is not well understood. Here, we address this issue from the perspective of metabolic state. Tilapias were more susceptible to Aeromonas sobria infection at 33°C than at 18°C, which is associated with differential metabolism of the fish. Compared to the metabolome of tilapia at 18°C, the metabolome at 33°C was characterized with increased an tricarboxylic acid cycle and a reduced level of myo-inositol which represent the most impactful pathway and crucial biomarker, respectively. These alterations were accompanied with the elevated transcriptional level of 10 innate immune genes with infection time, where il-1b, il-6, il-8, and il-10 exhibited a higher expression at 33°C than at 18°C and was attenuated by exogenous myo-inositol in both groups. Interestingly, exogenous myo-inositol inactivated the elevated TCA cycle via inhibiting the enzymatic activity of succinate dehydrogenase and malate dehydrogenase. Thus, tilapias showed a higher survival ability at 33°C. Our study reveals a previously unknown relationship among water temperature, metabolic state, and innate immunity and establishes a novel approach to eliminate bacterial pathogens in tilapia at higher water temperature. Frontiers Media S.A. 2021-09-10 /pmc/articles/PMC8462736/ /pubmed/34566956 http://dx.doi.org/10.3389/fimmu.2021.682724 Text en Copyright © 2021 Yang, Jiang, Peng and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Yang, Man-jun
Jiang, Ming
Peng, Xuan-xian
Li, Hui
Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature
title Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature
title_full Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature
title_fullStr Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature
title_full_unstemmed Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature
title_short Myo-Inositol Restores Tilapia’s Ability Against Infection by Aeromonas sobria in Higher Water Temperature
title_sort myo-inositol restores tilapia’s ability against infection by aeromonas sobria in higher water temperature
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8462736/
https://www.ncbi.nlm.nih.gov/pubmed/34566956
http://dx.doi.org/10.3389/fimmu.2021.682724
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