UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternati...

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Detalles Bibliográficos
Autores principales: Alcántara, Roberto, Peñaranda, Katherin, Mendoza-Rojas, Gabriel, Nakamoto, Jose A., Dueñas, Eva, Alvarez, Daniela, Adaui, Vanessa, Milón, Pohl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463329/
https://www.ncbi.nlm.nih.gov/pubmed/34604812
http://dx.doi.org/10.1016/j.xpro.2021.100878
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author Alcántara, Roberto
Peñaranda, Katherin
Mendoza-Rojas, Gabriel
Nakamoto, Jose A.
Dueñas, Eva
Alvarez, Daniela
Adaui, Vanessa
Milón, Pohl
author_facet Alcántara, Roberto
Peñaranda, Katherin
Mendoza-Rojas, Gabriel
Nakamoto, Jose A.
Dueñas, Eva
Alvarez, Daniela
Adaui, Vanessa
Milón, Pohl
author_sort Alcántara, Roberto
collection PubMed
description Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.
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spelling pubmed-84633292021-09-27 UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection Alcántara, Roberto Peñaranda, Katherin Mendoza-Rojas, Gabriel Nakamoto, Jose A. Dueñas, Eva Alvarez, Daniela Adaui, Vanessa Milón, Pohl STAR Protoc Protocol Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021. Elsevier 2021-09-25 /pmc/articles/PMC8463329/ /pubmed/34604812 http://dx.doi.org/10.1016/j.xpro.2021.100878 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Alcántara, Roberto
Peñaranda, Katherin
Mendoza-Rojas, Gabriel
Nakamoto, Jose A.
Dueñas, Eva
Alvarez, Daniela
Adaui, Vanessa
Milón, Pohl
UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_full UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_fullStr UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_full_unstemmed UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_short UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
title_sort uncovid: a versatile, low-cost, and open-source protocol for sars-cov-2 rna detection
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463329/
https://www.ncbi.nlm.nih.gov/pubmed/34604812
http://dx.doi.org/10.1016/j.xpro.2021.100878
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