UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternati...

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Detalles Bibliográficos
Autores principales: Alcántara, Roberto, Peñaranda, Katherin, Mendoza-Rojas, Gabriel, Nakamoto, Jose A., Dueñas, Eva, Alvarez, Daniela, Adaui, Vanessa, Milón, Pohl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463329/
https://www.ncbi.nlm.nih.gov/pubmed/34604812
http://dx.doi.org/10.1016/j.xpro.2021.100878

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463329/
https://www.ncbi.nlm.nih.gov/pubmed/34604812
http://dx.doi.org/10.1016/j.xpro.2021.100878

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