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Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of the technology, efficient and direct intracellular delivery of Cas9 ribonucleoprotein (RNP) complexes in...

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Detalles Bibliográficos
Autores principales:	Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, Van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C., Brans, Toon, de Jong, Olivier Gerrit, Maas-Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, De Smedt, Stefaan C., Vandekerckhove, Bart, Raemdonck, Koen, Braeckmans, Kevin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2021
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463438/ https://www.ncbi.nlm.nih.gov/pubmed/34589287 http://dx.doi.org/10.1016/j.omtn.2021.08.014

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