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Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats

BACKGROUND & OBJECTIVE: Reproductive toxicity of cadmium (Cd) as an environmental toxicant has been proved in animals and humans. Exposure to Cd impairs testes organs and can reduce male fertility. The present study was designed to investigate the spectrum of histopathological changes in testicu...

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Autores principales: Shojaeepour, Saeedeh, Dabiri, Shahriar, Dabiri, Bahram, Imani, Masoud, Fekri Soofi Abadi, Maryam, Hashemi, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Pathology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463756/
https://www.ncbi.nlm.nih.gov/pubmed/34567182
http://dx.doi.org/10.30699/IJP.20201.130581.2443
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author Shojaeepour, Saeedeh
Dabiri, Shahriar
Dabiri, Bahram
Imani, Masoud
Fekri Soofi Abadi, Maryam
Hashemi, Fatemeh
author_facet Shojaeepour, Saeedeh
Dabiri, Shahriar
Dabiri, Bahram
Imani, Masoud
Fekri Soofi Abadi, Maryam
Hashemi, Fatemeh
author_sort Shojaeepour, Saeedeh
collection PubMed
description BACKGROUND & OBJECTIVE: Reproductive toxicity of cadmium (Cd) as an environmental toxicant has been proved in animals and humans. Exposure to Cd impairs testes organs and can reduce male fertility. The present study was designed to investigate the spectrum of histopathological changes in testicular tissue focusing on Sertoli cells in rats following Cd intoxication. METHODS: In the present experiment, acute testicular toxicity was induced by an intraperitoneal injection of 1.2 mg/kg CdCl(2) to the animals in the test group, while the control group received normal saline. After 52 days, the animals were euthanized, and testicular tissue was stained by Hematoxylin and Eosin. In addition, immunohistochemical staining was performed on Sertoli cells for Wilms' Tumor, Melan-A, and CD99 to evaluate histopathological changes. RESULTS: Cd caused significant alterations in seminiferous tubules with varying effects on the patterns of spermatozoa production. These histopathological changes were significantly higher in the Cd group, compared to the control group. CONCLUSION: The Cd-induced stepwise spectrum changes included sloughing, disorganization, hypospermatogenesis, spermatic cell arrest, germ cell hypoplasia, Sertoli cell-only pattern, fibro-hyalinized seminiferous tubules, and calcification. Sertoli cells accumulated and created multinucleated giant cells in the seminiferous tubules during the atrophic process, which could be dependent upon Sertoli cells viability and function.
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spelling pubmed-84637562021-09-25 Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats Shojaeepour, Saeedeh Dabiri, Shahriar Dabiri, Bahram Imani, Masoud Fekri Soofi Abadi, Maryam Hashemi, Fatemeh Iran J Pathol Original Article BACKGROUND & OBJECTIVE: Reproductive toxicity of cadmium (Cd) as an environmental toxicant has been proved in animals and humans. Exposure to Cd impairs testes organs and can reduce male fertility. The present study was designed to investigate the spectrum of histopathological changes in testicular tissue focusing on Sertoli cells in rats following Cd intoxication. METHODS: In the present experiment, acute testicular toxicity was induced by an intraperitoneal injection of 1.2 mg/kg CdCl(2) to the animals in the test group, while the control group received normal saline. After 52 days, the animals were euthanized, and testicular tissue was stained by Hematoxylin and Eosin. In addition, immunohistochemical staining was performed on Sertoli cells for Wilms' Tumor, Melan-A, and CD99 to evaluate histopathological changes. RESULTS: Cd caused significant alterations in seminiferous tubules with varying effects on the patterns of spermatozoa production. These histopathological changes were significantly higher in the Cd group, compared to the control group. CONCLUSION: The Cd-induced stepwise spectrum changes included sloughing, disorganization, hypospermatogenesis, spermatic cell arrest, germ cell hypoplasia, Sertoli cell-only pattern, fibro-hyalinized seminiferous tubules, and calcification. Sertoli cells accumulated and created multinucleated giant cells in the seminiferous tubules during the atrophic process, which could be dependent upon Sertoli cells viability and function. Iranian Society of Pathology 2021 2021-07-06 /pmc/articles/PMC8463756/ /pubmed/34567182 http://dx.doi.org/10.30699/IJP.20201.130581.2443 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution- 4.0 International License, (https://creativecommons.org/licenses/by/4.0/) which permits Share, copy and redistribution of the material in any medium or format or adapt, remix, transform, and build upon the material for any purpose, even commercially
spellingShingle Original Article
Shojaeepour, Saeedeh
Dabiri, Shahriar
Dabiri, Bahram
Imani, Masoud
Fekri Soofi Abadi, Maryam
Hashemi, Fatemeh
Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats
title Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats
title_full Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats
title_fullStr Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats
title_full_unstemmed Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats
title_short Histopathological Findings of Testicular Tissue Following Cadmium Toxicity in Rats
title_sort histopathological findings of testicular tissue following cadmium toxicity in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463756/
https://www.ncbi.nlm.nih.gov/pubmed/34567182
http://dx.doi.org/10.30699/IJP.20201.130581.2443
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