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Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection

The study was conducted to evaluate the effects of graded levels of Eimeria maxima challenge on endogenous loss, apparent ileal digestibility (AID), and standard ileal digestibility (SID) of amino acids. A total of 768 fourteen-day-old male Cobb 500 broiler chickens were randomly allocated into 64 b...

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Autores principales: Teng, Po-Yun, Yadav, Sudhir, Shi, Hanyi, Kim, Woo Kyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463777/
https://www.ncbi.nlm.nih.gov/pubmed/34547620
http://dx.doi.org/10.1016/j.psj.2021.101426
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author Teng, Po-Yun
Yadav, Sudhir
Shi, Hanyi
Kim, Woo Kyun
author_facet Teng, Po-Yun
Yadav, Sudhir
Shi, Hanyi
Kim, Woo Kyun
author_sort Teng, Po-Yun
collection PubMed
description The study was conducted to evaluate the effects of graded levels of Eimeria maxima challenge on endogenous loss, apparent ileal digestibility (AID), and standard ileal digestibility (SID) of amino acids. A total of 768 fourteen-day-old male Cobb 500 broiler chickens were randomly allocated into 64 battery cages. Apart from the regular corn-soybean based diet, the nitrogen-free diet (NFD) was formulated to determine the endogenous loss of amino acids. One-half of the birds (32 cages) were fed the NFD, and another half fed the regular diet from d 14 to 20. Both groups were further assigned to 4 treatments (nonchallenged control or three levels of challenge doses) with 8 replicate cages. The challenge doses were: the low challenge dose (Low) with E. maxima 12,500 oocysts, the medium challenge dose (Medium) with 25,000 E. maxima oocysts, and the high challenge dose (High) with 50,000 E. maxima oocysts. At 6 d postinfection, ileal digesta samples were collected and the intestinal lesion score were recorded. The results indicated a significant linear increase of endogenous amino acid flow in response to the graded E. maxima challenge. Moreover, the AID and SID of amino acids were linearly reduced due to the increasing challenge dose. The study demonstrated that NFD significantly reduced lesion scores, underestimating the true endogenous losses of birds fed regular diets. Even though the endogenous loss of amino acids was underestimated, they were linearly increased in response to the graded E. maxima challenge. In conclusion, the higher Eimeria dose birds were challenged with, the more endogenous amino acids were released into the intestine and the lower dietary nutrients were digested and absorbed by broiler chickens.
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spelling pubmed-84637772021-10-01 Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection Teng, Po-Yun Yadav, Sudhir Shi, Hanyi Kim, Woo Kyun Poult Sci METABOLISM AND NUTRITION The study was conducted to evaluate the effects of graded levels of Eimeria maxima challenge on endogenous loss, apparent ileal digestibility (AID), and standard ileal digestibility (SID) of amino acids. A total of 768 fourteen-day-old male Cobb 500 broiler chickens were randomly allocated into 64 battery cages. Apart from the regular corn-soybean based diet, the nitrogen-free diet (NFD) was formulated to determine the endogenous loss of amino acids. One-half of the birds (32 cages) were fed the NFD, and another half fed the regular diet from d 14 to 20. Both groups were further assigned to 4 treatments (nonchallenged control or three levels of challenge doses) with 8 replicate cages. The challenge doses were: the low challenge dose (Low) with E. maxima 12,500 oocysts, the medium challenge dose (Medium) with 25,000 E. maxima oocysts, and the high challenge dose (High) with 50,000 E. maxima oocysts. At 6 d postinfection, ileal digesta samples were collected and the intestinal lesion score were recorded. The results indicated a significant linear increase of endogenous amino acid flow in response to the graded E. maxima challenge. Moreover, the AID and SID of amino acids were linearly reduced due to the increasing challenge dose. The study demonstrated that NFD significantly reduced lesion scores, underestimating the true endogenous losses of birds fed regular diets. Even though the endogenous loss of amino acids was underestimated, they were linearly increased in response to the graded E. maxima challenge. In conclusion, the higher Eimeria dose birds were challenged with, the more endogenous amino acids were released into the intestine and the lower dietary nutrients were digested and absorbed by broiler chickens. Elsevier 2021-08-13 /pmc/articles/PMC8463777/ /pubmed/34547620 http://dx.doi.org/10.1016/j.psj.2021.101426 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle METABOLISM AND NUTRITION
Teng, Po-Yun
Yadav, Sudhir
Shi, Hanyi
Kim, Woo Kyun
Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection
title Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection
title_full Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection
title_fullStr Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection
title_full_unstemmed Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection
title_short Evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of E. maxima infection
title_sort evaluating endogenous loss and standard ileal digestibility of amino acids in response to the graded severity levels of e. maxima infection
topic METABOLISM AND NUTRITION
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463777/
https://www.ncbi.nlm.nih.gov/pubmed/34547620
http://dx.doi.org/10.1016/j.psj.2021.101426
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