Novel human cell expression method reveals the role and prevalence of posttranslational modification in nonmuscle tropomyosins

Biochemical studies require large quantities of proteins, which are typically obtained using bacterial overexpression. However, the folding machinery in bacteria is inadequate for expressing many mammalian proteins, which additionally undergo posttranslational modifications (PTMs) that bacteria, yea...

Descripción completa

Detalles Bibliográficos
Autores principales: Carman, Peter J., Barrie, Kyle R., Dominguez, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463859/
https://www.ncbi.nlm.nih.gov/pubmed/34478714
http://dx.doi.org/10.1016/j.jbc.2021.101154

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463859/
https://www.ncbi.nlm.nih.gov/pubmed/34478714
http://dx.doi.org/10.1016/j.jbc.2021.101154

Ejemplares similares