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Novel human cell expression method reveals the role and prevalence of posttranslational modification in nonmuscle tropomyosins
Biochemical studies require large quantities of proteins, which are typically obtained using bacterial overexpression. However, the folding machinery in bacteria is inadequate for expressing many mammalian proteins, which additionally undergo posttranslational modifications (PTMs) that bacteria, yea...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463859/ https://www.ncbi.nlm.nih.gov/pubmed/34478714 http://dx.doi.org/10.1016/j.jbc.2021.101154 |