Phase separation dynamics of the C. elegans PGL-1 P granule protein in oocytes are sensitive to heat stress

Phase separation has emerged as a widespread process of organizing the cytoplasm of diverse eukaryotic cells. In C. elegans oocytes, several RNA binding proteins are condensed into germ granules called P granules. Prior studies studying the phase transitions of RNA binding proteins in response to increased temperature have suggested that PGL-1 decondenses in oocytes in response to heat. Here, we confirm this finding with a new reporter strain and demonstrate the sensitivity of PGL-1 to temperature changes.