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Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription
Non-coding transcription is an important determinant of heterochromatin formation. In Arabidopsis thaliana a specialized RNA polymerase V (Pol V) transcribes pervasively and produces long non-coding RNAs. These transcripts work with small interfering RNA to facilitate locus-specific establishment of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464056/ https://www.ncbi.nlm.nih.gov/pubmed/34469565 http://dx.doi.org/10.1093/nar/gkab746 |
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author | Rothi, M Hafiz Tsuzuki, Masayuki Sethuraman, Shriya Wierzbicki, Andrzej T |
author_facet | Rothi, M Hafiz Tsuzuki, Masayuki Sethuraman, Shriya Wierzbicki, Andrzej T |
author_sort | Rothi, M Hafiz |
collection | PubMed |
description | Non-coding transcription is an important determinant of heterochromatin formation. In Arabidopsis thaliana a specialized RNA polymerase V (Pol V) transcribes pervasively and produces long non-coding RNAs. These transcripts work with small interfering RNA to facilitate locus-specific establishment of RNA-directed DNA methylation (RdDM). Subsequent maintenance of RdDM is associated with elevated levels of Pol V transcription. However, the impact of DNA methylation on Pol V transcription remained unresolved. We found that DNA methylation strongly enhances Pol V transcription. The level of Pol V transcription is reduced in mutants defective in RdDM components working downstream of Pol V, indicating that RdDM is maintained by a mutual reinforcement of DNA methylation and Pol V transcription. Pol V transcription is affected only on loci that lose DNA methylation in all sequence contexts in a particular mutant, including mutants lacking maintenance DNA methyltransferases, which suggests that RdDM works in a complex crosstalk with other silencing pathways. |
format | Online Article Text |
id | pubmed-8464056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-84640562021-09-27 Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription Rothi, M Hafiz Tsuzuki, Masayuki Sethuraman, Shriya Wierzbicki, Andrzej T Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Non-coding transcription is an important determinant of heterochromatin formation. In Arabidopsis thaliana a specialized RNA polymerase V (Pol V) transcribes pervasively and produces long non-coding RNAs. These transcripts work with small interfering RNA to facilitate locus-specific establishment of RNA-directed DNA methylation (RdDM). Subsequent maintenance of RdDM is associated with elevated levels of Pol V transcription. However, the impact of DNA methylation on Pol V transcription remained unresolved. We found that DNA methylation strongly enhances Pol V transcription. The level of Pol V transcription is reduced in mutants defective in RdDM components working downstream of Pol V, indicating that RdDM is maintained by a mutual reinforcement of DNA methylation and Pol V transcription. Pol V transcription is affected only on loci that lose DNA methylation in all sequence contexts in a particular mutant, including mutants lacking maintenance DNA methyltransferases, which suggests that RdDM works in a complex crosstalk with other silencing pathways. Oxford University Press 2021-09-01 /pmc/articles/PMC8464056/ /pubmed/34469565 http://dx.doi.org/10.1093/nar/gkab746 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene regulation, Chromatin and Epigenetics Rothi, M Hafiz Tsuzuki, Masayuki Sethuraman, Shriya Wierzbicki, Andrzej T Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription |
title | Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription |
title_full | Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription |
title_fullStr | Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription |
title_full_unstemmed | Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription |
title_short | Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription |
title_sort | reinforcement of transcriptional silencing by a positive feedback between dna methylation and non-coding transcription |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464056/ https://www.ncbi.nlm.nih.gov/pubmed/34469565 http://dx.doi.org/10.1093/nar/gkab746 |
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