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Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana
In the current meiotic recombination initiation model, the SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and the plant specific DFO are required f...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464057/ https://www.ncbi.nlm.nih.gov/pubmed/34458909 http://dx.doi.org/10.1093/nar/gkab715 |
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author | Vrielynck, Nathalie Schneider, Katja Rodriguez, Marion Sims, Jason Chambon, Aurélie Hurel, Aurélie De Muyt, Arnaud Ronceret, Arnaud Krsicka, Ondrej Mézard, Christine Schlögelhofer, Peter Grelon, Mathilde |
author_facet | Vrielynck, Nathalie Schneider, Katja Rodriguez, Marion Sims, Jason Chambon, Aurélie Hurel, Aurélie De Muyt, Arnaud Ronceret, Arnaud Krsicka, Ondrej Mézard, Christine Schlögelhofer, Peter Grelon, Mathilde |
author_sort | Vrielynck, Nathalie |
collection | PubMed |
description | In the current meiotic recombination initiation model, the SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and the plant specific DFO are required for meiotic DSB formation. Here we show that (i) MTOPVIB and PRD1 provide the link between the catalytic sub-complex and the other DSB proteins, (ii) PRD3/AtMER2, while localized to the axis, does not assemble a canonical pre-DSB complex but establishes a direct link between the DSB-forming and resection machineries, (iii) DFO controls MTOPVIB foci formation and is part of a divergent RMM-like complex including PHS1/AtREC114 and PRD2/AtMEI4 but not PRD3/AtMER2, (iv) PHS1/AtREC114 is absolutely unnecessary for DSB formation despite having a conserved position within the DSB protein network and (v) MTOPVIB and PRD2/AtMEI4 interact directly with chromosome axis proteins to anchor the meiotic DSB machinery to the axis. |
format | Online Article Text |
id | pubmed-8464057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-84640572021-09-27 Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana Vrielynck, Nathalie Schneider, Katja Rodriguez, Marion Sims, Jason Chambon, Aurélie Hurel, Aurélie De Muyt, Arnaud Ronceret, Arnaud Krsicka, Ondrej Mézard, Christine Schlögelhofer, Peter Grelon, Mathilde Nucleic Acids Res Genome Integrity, Repair and Replication In the current meiotic recombination initiation model, the SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and the plant specific DFO are required for meiotic DSB formation. Here we show that (i) MTOPVIB and PRD1 provide the link between the catalytic sub-complex and the other DSB proteins, (ii) PRD3/AtMER2, while localized to the axis, does not assemble a canonical pre-DSB complex but establishes a direct link between the DSB-forming and resection machineries, (iii) DFO controls MTOPVIB foci formation and is part of a divergent RMM-like complex including PHS1/AtREC114 and PRD2/AtMEI4 but not PRD3/AtMER2, (iv) PHS1/AtREC114 is absolutely unnecessary for DSB formation despite having a conserved position within the DSB protein network and (v) MTOPVIB and PRD2/AtMEI4 interact directly with chromosome axis proteins to anchor the meiotic DSB machinery to the axis. Oxford University Press 2021-08-30 /pmc/articles/PMC8464057/ /pubmed/34458909 http://dx.doi.org/10.1093/nar/gkab715 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Genome Integrity, Repair and Replication Vrielynck, Nathalie Schneider, Katja Rodriguez, Marion Sims, Jason Chambon, Aurélie Hurel, Aurélie De Muyt, Arnaud Ronceret, Arnaud Krsicka, Ondrej Mézard, Christine Schlögelhofer, Peter Grelon, Mathilde Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana |
title | Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana |
title_full | Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana |
title_fullStr | Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana |
title_full_unstemmed | Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana |
title_short | Conservation and divergence of meiotic DNA double strand break forming mechanisms in Arabidopsis thaliana |
title_sort | conservation and divergence of meiotic dna double strand break forming mechanisms in arabidopsis thaliana |
topic | Genome Integrity, Repair and Replication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464057/ https://www.ncbi.nlm.nih.gov/pubmed/34458909 http://dx.doi.org/10.1093/nar/gkab715 |
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