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Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo
INTRODUCTION: Vitiligo pathogenesis is complicated, and several possibilities were suggested. However, it is well-known that the metabolism of pigments plays a significant role in the pathogenicity of the disease. OBJECTIVES: We explored the role of amino acids in vitiligo using targeted metabolomic...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464575/ https://www.ncbi.nlm.nih.gov/pubmed/34562159 http://dx.doi.org/10.1007/s11306-021-01843-x |
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author | Marzabani, Rezvan Rezadoost, Hassan Choopanian, Peyman Kolahdooz, Sima Mozafari, Nikoo Mirzaie, Mehdi Karimi, Mehrdad Nieminen, Anni I. Jafari, Mohieddin |
author_facet | Marzabani, Rezvan Rezadoost, Hassan Choopanian, Peyman Kolahdooz, Sima Mozafari, Nikoo Mirzaie, Mehdi Karimi, Mehrdad Nieminen, Anni I. Jafari, Mohieddin |
author_sort | Marzabani, Rezvan |
collection | PubMed |
description | INTRODUCTION: Vitiligo pathogenesis is complicated, and several possibilities were suggested. However, it is well-known that the metabolism of pigments plays a significant role in the pathogenicity of the disease. OBJECTIVES: We explored the role of amino acids in vitiligo using targeted metabolomics. METHODS: The amino acid profile was studied in plasma using liquid chromatography. First, 22 amino acids were derivatized and precisely determined. Next, the concentrations of the amino acids and the molar ratios were calculated in 31 patients and 34 healthy individuals. RESULTS: The differential concentrations of amino acids were analyzed and eight amino acids, i.e., cysteine, arginine, lysine, ornithine, proline, glutamic acid, histidine, and glycine were observed differentially. The ratios of cysteine, glutamic acid, and proline increased significantly in Vitiligo patients, whereas arginine, lysine, ornithine, glycine, and histidine decreased significantly compared to healthy individuals. Considering the percentage of skin area, we also showed that glutamic acid significantly has a higher amount in patients with less than 25% involvement compared to others. Finally, cysteine and lysine are considered promising candidates for diagnosing and developing the disorder with high accuracy (0.96). CONCLUSION: The findings are consistent with the previously illustrated mechanism of Vitiligo, such as production deficiency in melanin and an increase in immune activity and oxidative stress. Furthermore, new evidence was provided by using amino acids profile toward the pathogenicity of the disorder. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11306-021-01843-x |
format | Online Article Text |
id | pubmed-8464575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-84645752021-10-08 Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo Marzabani, Rezvan Rezadoost, Hassan Choopanian, Peyman Kolahdooz, Sima Mozafari, Nikoo Mirzaie, Mehdi Karimi, Mehrdad Nieminen, Anni I. Jafari, Mohieddin Metabolomics Original Article INTRODUCTION: Vitiligo pathogenesis is complicated, and several possibilities were suggested. However, it is well-known that the metabolism of pigments plays a significant role in the pathogenicity of the disease. OBJECTIVES: We explored the role of amino acids in vitiligo using targeted metabolomics. METHODS: The amino acid profile was studied in plasma using liquid chromatography. First, 22 amino acids were derivatized and precisely determined. Next, the concentrations of the amino acids and the molar ratios were calculated in 31 patients and 34 healthy individuals. RESULTS: The differential concentrations of amino acids were analyzed and eight amino acids, i.e., cysteine, arginine, lysine, ornithine, proline, glutamic acid, histidine, and glycine were observed differentially. The ratios of cysteine, glutamic acid, and proline increased significantly in Vitiligo patients, whereas arginine, lysine, ornithine, glycine, and histidine decreased significantly compared to healthy individuals. Considering the percentage of skin area, we also showed that glutamic acid significantly has a higher amount in patients with less than 25% involvement compared to others. Finally, cysteine and lysine are considered promising candidates for diagnosing and developing the disorder with high accuracy (0.96). CONCLUSION: The findings are consistent with the previously illustrated mechanism of Vitiligo, such as production deficiency in melanin and an increase in immune activity and oxidative stress. Furthermore, new evidence was provided by using amino acids profile toward the pathogenicity of the disorder. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11306-021-01843-x Springer US 2021-09-25 2021 /pmc/articles/PMC8464575/ /pubmed/34562159 http://dx.doi.org/10.1007/s11306-021-01843-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Marzabani, Rezvan Rezadoost, Hassan Choopanian, Peyman Kolahdooz, Sima Mozafari, Nikoo Mirzaie, Mehdi Karimi, Mehrdad Nieminen, Anni I. Jafari, Mohieddin Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo |
title | Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo |
title_full | Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo |
title_fullStr | Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo |
title_full_unstemmed | Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo |
title_short | Metabolomic signature of amino acids in plasma of patients with non-segmental Vitiligo |
title_sort | metabolomic signature of amino acids in plasma of patients with non-segmental vitiligo |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464575/ https://www.ncbi.nlm.nih.gov/pubmed/34562159 http://dx.doi.org/10.1007/s11306-021-01843-x |
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