Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B

In order to study how polyphenols and vitamin C (vitC) together affect protein aggregation to amyloid fibrils, we performed similar in vitro studies as before using stefin B as a model and a potentially amyloid-forming protein (it aggregates upon overexpression, under stressful conditions and some p...

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Autores principales: Jahić Mujkić, Alma, Tušek Žnidarič, Magda, Berbić, Selma, Žerovnik, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464686/
https://www.ncbi.nlm.nih.gov/pubmed/34573102
http://dx.doi.org/10.3390/antiox10091471
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author Jahić Mujkić, Alma
Tušek Žnidarič, Magda
Berbić, Selma
Žerovnik, Eva
author_facet Jahić Mujkić, Alma
Tušek Žnidarič, Magda
Berbić, Selma
Žerovnik, Eva
author_sort Jahić Mujkić, Alma
collection PubMed
description In order to study how polyphenols and vitamin C (vitC) together affect protein aggregation to amyloid fibrils, we performed similar in vitro studies as before using stefin B as a model and a potentially amyloid-forming protein (it aggregates upon overexpression, under stressful conditions and some progressive myoclonus epilepsy of tape 1—EPM1-missense mutations). In addition to the chosen polyphenol, this time, we added a proven antioxidant concentration of 0.5 mM vitC into the fibrillation mixture and varied concentrations of resveratrol, quercetin, and curcumin. Synergy with vitC was observed with curcumin and quercetin.
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spelling pubmed-84646862021-09-27 Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B Jahić Mujkić, Alma Tušek Žnidarič, Magda Berbić, Selma Žerovnik, Eva Antioxidants (Basel) Article In order to study how polyphenols and vitamin C (vitC) together affect protein aggregation to amyloid fibrils, we performed similar in vitro studies as before using stefin B as a model and a potentially amyloid-forming protein (it aggregates upon overexpression, under stressful conditions and some progressive myoclonus epilepsy of tape 1—EPM1-missense mutations). In addition to the chosen polyphenol, this time, we added a proven antioxidant concentration of 0.5 mM vitC into the fibrillation mixture and varied concentrations of resveratrol, quercetin, and curcumin. Synergy with vitC was observed with curcumin and quercetin. MDPI 2021-09-15 /pmc/articles/PMC8464686/ /pubmed/34573102 http://dx.doi.org/10.3390/antiox10091471 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jahić Mujkić, Alma
Tušek Žnidarič, Magda
Berbić, Selma
Žerovnik, Eva
Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
title Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
title_full Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
title_fullStr Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
title_full_unstemmed Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
title_short Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
title_sort synergy of the inhibitory action of polyphenols plus vitamin c on amyloid fibril formation: case study of human stefin b
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464686/
https://www.ncbi.nlm.nih.gov/pubmed/34573102
http://dx.doi.org/10.3390/antiox10091471
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