Cargando…

Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus

Lactobacillus acidophilus is a common kind of lactic acid bacteria usually found in the human gastrointestinal tract, oral cavity, vagina, and various fermented foods. At present, many studies have focused on the probiotic function and industrial application of L. acidophilus. Additionally, dozens o...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Zheng, Zhou, Xingya, Stanton, Catherine, Ross, Reynolds Paul, Zhao, Jianxin, Zhang, Hao, Yang, Bo, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464880/
https://www.ncbi.nlm.nih.gov/pubmed/34576887
http://dx.doi.org/10.3390/microorganisms9091992
_version_ 1784572727753965568
author Huang, Zheng
Zhou, Xingya
Stanton, Catherine
Ross, Reynolds Paul
Zhao, Jianxin
Zhang, Hao
Yang, Bo
Chen, Wei
author_facet Huang, Zheng
Zhou, Xingya
Stanton, Catherine
Ross, Reynolds Paul
Zhao, Jianxin
Zhang, Hao
Yang, Bo
Chen, Wei
author_sort Huang, Zheng
collection PubMed
description Lactobacillus acidophilus is a common kind of lactic acid bacteria usually found in the human gastrointestinal tract, oral cavity, vagina, and various fermented foods. At present, many studies have focused on the probiotic function and industrial application of L. acidophilus. Additionally, dozens of L. acidophilus strains have been genome sequenced, but there has been no research to compare them at the genomic level. In this study, 46 strains of L. acidophilus were performed comparative analyses to explore their genetic diversity. The results showed that all the L. acidophilus strains were divided into two clusters based on ANI values, phylogenetic analysis and whole genome comparison, due to the difference of their predicted gene composition of bacteriocin operon, CRISPR-Cas systems and prophages mainly. Additionally, L. acidophilus was a pan-genome open species with a difference in carbohydrates utilization, antibiotic resistance, EPS operon, surface layer protein operon and other functional gene composition. This work provides a better understanding of L. acidophilus from a genetic perspective, and offers a frame for the biotechnological potentiality of this species.
format Online
Article
Text
id pubmed-8464880
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84648802021-09-27 Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus Huang, Zheng Zhou, Xingya Stanton, Catherine Ross, Reynolds Paul Zhao, Jianxin Zhang, Hao Yang, Bo Chen, Wei Microorganisms Article Lactobacillus acidophilus is a common kind of lactic acid bacteria usually found in the human gastrointestinal tract, oral cavity, vagina, and various fermented foods. At present, many studies have focused on the probiotic function and industrial application of L. acidophilus. Additionally, dozens of L. acidophilus strains have been genome sequenced, but there has been no research to compare them at the genomic level. In this study, 46 strains of L. acidophilus were performed comparative analyses to explore their genetic diversity. The results showed that all the L. acidophilus strains were divided into two clusters based on ANI values, phylogenetic analysis and whole genome comparison, due to the difference of their predicted gene composition of bacteriocin operon, CRISPR-Cas systems and prophages mainly. Additionally, L. acidophilus was a pan-genome open species with a difference in carbohydrates utilization, antibiotic resistance, EPS operon, surface layer protein operon and other functional gene composition. This work provides a better understanding of L. acidophilus from a genetic perspective, and offers a frame for the biotechnological potentiality of this species. MDPI 2021-09-20 /pmc/articles/PMC8464880/ /pubmed/34576887 http://dx.doi.org/10.3390/microorganisms9091992 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Zheng
Zhou, Xingya
Stanton, Catherine
Ross, Reynolds Paul
Zhao, Jianxin
Zhang, Hao
Yang, Bo
Chen, Wei
Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus
title Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus
title_full Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus
title_fullStr Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus
title_full_unstemmed Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus
title_short Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus
title_sort comparative genomics and specific functional characteristics analysis of lactobacillus acidophilus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464880/
https://www.ncbi.nlm.nih.gov/pubmed/34576887
http://dx.doi.org/10.3390/microorganisms9091992
work_keys_str_mv AT huangzheng comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus
AT zhouxingya comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus
AT stantoncatherine comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus
AT rossreynoldspaul comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus
AT zhaojianxin comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus
AT zhanghao comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus
AT yangbo comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus
AT chenwei comparativegenomicsandspecificfunctionalcharacteristicsanalysisoflactobacillusacidophilus