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Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats

Previous experiments in animals and humans show that shifts in microbiota and its metabolites are linked to hypertension. The present study investigates whether doxycycline (DOX, a broad-spectrum tetracycline antibiotic) improves dysbiosis, prevent cardiovascular pathology and attenuate hypertension...

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Autores principales: Robles-Vera, Iñaki, de la Visitación, Néstor, Toral, Marta, Sánchez, Manuel, Romero, Miguel, Gómez-Guzmán, Manuel, Vargas, Félix, Duarte, Juan, Jiménez, Rosario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464928/
https://www.ncbi.nlm.nih.gov/pubmed/34578849
http://dx.doi.org/10.3390/nu13092971
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author Robles-Vera, Iñaki
de la Visitación, Néstor
Toral, Marta
Sánchez, Manuel
Romero, Miguel
Gómez-Guzmán, Manuel
Vargas, Félix
Duarte, Juan
Jiménez, Rosario
author_facet Robles-Vera, Iñaki
de la Visitación, Néstor
Toral, Marta
Sánchez, Manuel
Romero, Miguel
Gómez-Guzmán, Manuel
Vargas, Félix
Duarte, Juan
Jiménez, Rosario
author_sort Robles-Vera, Iñaki
collection PubMed
description Previous experiments in animals and humans show that shifts in microbiota and its metabolites are linked to hypertension. The present study investigates whether doxycycline (DOX, a broad-spectrum tetracycline antibiotic) improves dysbiosis, prevent cardiovascular pathology and attenuate hypertension in deoxycorticosterone acetate (DOCA)-salt rats, a renin-independent model of hypertension. Male Wistar rats were randomly assigned to three groups: control, DOCA-salt hypertensive rats, DOCA-salt treated with DOX for 4 weeks. DOX decreased systolic blood pressure, improving endothelial dysfunction and reducing aortic oxidative stress and inflammation. DOX decreased lactate-producing bacterial population and plasma lactate levels, improved gut barrier integrity, normalized endotoxemia, plasma noradrenaline levels and restored the Treg content in aorta. These data demonstrate that DOX through direct effects on gut microbiota and its non-microbial effects (anti-inflammatory and immunomodulatory) reduces endothelial dysfunction and the increase in blood pressure in this low-renin form of hypertension.
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spelling pubmed-84649282021-09-27 Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats Robles-Vera, Iñaki de la Visitación, Néstor Toral, Marta Sánchez, Manuel Romero, Miguel Gómez-Guzmán, Manuel Vargas, Félix Duarte, Juan Jiménez, Rosario Nutrients Article Previous experiments in animals and humans show that shifts in microbiota and its metabolites are linked to hypertension. The present study investigates whether doxycycline (DOX, a broad-spectrum tetracycline antibiotic) improves dysbiosis, prevent cardiovascular pathology and attenuate hypertension in deoxycorticosterone acetate (DOCA)-salt rats, a renin-independent model of hypertension. Male Wistar rats were randomly assigned to three groups: control, DOCA-salt hypertensive rats, DOCA-salt treated with DOX for 4 weeks. DOX decreased systolic blood pressure, improving endothelial dysfunction and reducing aortic oxidative stress and inflammation. DOX decreased lactate-producing bacterial population and plasma lactate levels, improved gut barrier integrity, normalized endotoxemia, plasma noradrenaline levels and restored the Treg content in aorta. These data demonstrate that DOX through direct effects on gut microbiota and its non-microbial effects (anti-inflammatory and immunomodulatory) reduces endothelial dysfunction and the increase in blood pressure in this low-renin form of hypertension. MDPI 2021-08-26 /pmc/articles/PMC8464928/ /pubmed/34578849 http://dx.doi.org/10.3390/nu13092971 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Robles-Vera, Iñaki
de la Visitación, Néstor
Toral, Marta
Sánchez, Manuel
Romero, Miguel
Gómez-Guzmán, Manuel
Vargas, Félix
Duarte, Juan
Jiménez, Rosario
Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats
title Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats
title_full Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats
title_fullStr Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats
title_full_unstemmed Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats
title_short Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats
title_sort changes in gut microbiota induced by doxycycline influence in vascular function and development of hypertension in doca-salt rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464928/
https://www.ncbi.nlm.nih.gov/pubmed/34578849
http://dx.doi.org/10.3390/nu13092971
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