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Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2

Extracellular vesicles (EVs) are cell-released, nanometer-scaled, membrane-bound materials and contain diverse contents including proteins, small peptides, and nucleic acids. Once released, EVs can alter the microenvironment and regulate a myriad of cellular physiology components, including cell–cel...

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Autores principales: Park, Jae Hyun, Choi, Yuri, Lim, Chul-Woo, Park, Ji-Min, Yu, Shin-Hye, Kim, Yujin, Han, Hae Jung, Kim, Chun-Hyung, Song, Young-Sook, Kim, Chul, Yu, Seung Rok, Oh, Eun Young, Lee, Sang-Myeong, Moon, Jisook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465096/
https://www.ncbi.nlm.nih.gov/pubmed/34572043
http://dx.doi.org/10.3390/cells10092393
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author Park, Jae Hyun
Choi, Yuri
Lim, Chul-Woo
Park, Ji-Min
Yu, Shin-Hye
Kim, Yujin
Han, Hae Jung
Kim, Chun-Hyung
Song, Young-Sook
Kim, Chul
Yu, Seung Rok
Oh, Eun Young
Lee, Sang-Myeong
Moon, Jisook
author_facet Park, Jae Hyun
Choi, Yuri
Lim, Chul-Woo
Park, Ji-Min
Yu, Shin-Hye
Kim, Yujin
Han, Hae Jung
Kim, Chun-Hyung
Song, Young-Sook
Kim, Chul
Yu, Seung Rok
Oh, Eun Young
Lee, Sang-Myeong
Moon, Jisook
author_sort Park, Jae Hyun
collection PubMed
description Extracellular vesicles (EVs) are cell-released, nanometer-scaled, membrane-bound materials and contain diverse contents including proteins, small peptides, and nucleic acids. Once released, EVs can alter the microenvironment and regulate a myriad of cellular physiology components, including cell–cell communication, proliferation, differentiation, and immune responses against viral infection. Among the cargoes in the vesicles, small non-coding micro-RNAs (miRNAs) have received attention in that they can regulate the expression of a variety of human genes as well as external viral genes via binding to the complementary mRNAs. In this study, we tested the potential of EVs as therapeutic agents for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. First, we found that the mesenchymal stem-cell-derived EVs (MSC-EVs) enabled the rescue of the cytopathic effect of SARS-CoV-2 virus and the suppression of proinflammatory responses in the infected cells by inhibiting the viral replication. We found that these anti-viral responses were mediated by 17 miRNAs matching the rarely mutated, conserved 3′-untranslated regions (UTR) of the viral genome. The top five miRNAs highly expressed in the MSC-EVs, miR-92a-3p, miR-26a-5p, miR-23a-3p, miR-103a-3p, and miR-181a-5p, were tested. They were bound to the complemented sequence which led to the recovery of the cytopathic effects. These findings suggest that the MSC-EVs are a potential candidate for multiple variants of anti-SARS-CoV-2.
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spelling pubmed-84650962021-09-27 Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2 Park, Jae Hyun Choi, Yuri Lim, Chul-Woo Park, Ji-Min Yu, Shin-Hye Kim, Yujin Han, Hae Jung Kim, Chun-Hyung Song, Young-Sook Kim, Chul Yu, Seung Rok Oh, Eun Young Lee, Sang-Myeong Moon, Jisook Cells Article Extracellular vesicles (EVs) are cell-released, nanometer-scaled, membrane-bound materials and contain diverse contents including proteins, small peptides, and nucleic acids. Once released, EVs can alter the microenvironment and regulate a myriad of cellular physiology components, including cell–cell communication, proliferation, differentiation, and immune responses against viral infection. Among the cargoes in the vesicles, small non-coding micro-RNAs (miRNAs) have received attention in that they can regulate the expression of a variety of human genes as well as external viral genes via binding to the complementary mRNAs. In this study, we tested the potential of EVs as therapeutic agents for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. First, we found that the mesenchymal stem-cell-derived EVs (MSC-EVs) enabled the rescue of the cytopathic effect of SARS-CoV-2 virus and the suppression of proinflammatory responses in the infected cells by inhibiting the viral replication. We found that these anti-viral responses were mediated by 17 miRNAs matching the rarely mutated, conserved 3′-untranslated regions (UTR) of the viral genome. The top five miRNAs highly expressed in the MSC-EVs, miR-92a-3p, miR-26a-5p, miR-23a-3p, miR-103a-3p, and miR-181a-5p, were tested. They were bound to the complemented sequence which led to the recovery of the cytopathic effects. These findings suggest that the MSC-EVs are a potential candidate for multiple variants of anti-SARS-CoV-2. MDPI 2021-09-12 /pmc/articles/PMC8465096/ /pubmed/34572043 http://dx.doi.org/10.3390/cells10092393 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Jae Hyun
Choi, Yuri
Lim, Chul-Woo
Park, Ji-Min
Yu, Shin-Hye
Kim, Yujin
Han, Hae Jung
Kim, Chun-Hyung
Song, Young-Sook
Kim, Chul
Yu, Seung Rok
Oh, Eun Young
Lee, Sang-Myeong
Moon, Jisook
Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
title Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
title_full Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
title_fullStr Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
title_full_unstemmed Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
title_short Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
title_sort potential therapeutic effect of micrornas in extracellular vesicles from mesenchymal stem cells against sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465096/
https://www.ncbi.nlm.nih.gov/pubmed/34572043
http://dx.doi.org/10.3390/cells10092393
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