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C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study
P-glycoprotein encoded by the ABCB1 gene constitutes a molecular barrier in the small and large bowel epithelium, and its different expression may influence susceptibility to inflammatory bowel disease (IBD). We aimed to assess the contribution of the C3435T polymorphism to disease risk in the Polis...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465101/ https://www.ncbi.nlm.nih.gov/pubmed/34573401 http://dx.doi.org/10.3390/genes12091419 |
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author | Petryszyn, Paweł Dudkowiak, Robert Gruca, Agnieszka Jaźwińska-Tarnawska, Ewa Ekk-Cierniakowski, Paweł Poniewierka, Elżbieta Wiela-Hojeńska, Anna Głowacka, Krystyna |
author_facet | Petryszyn, Paweł Dudkowiak, Robert Gruca, Agnieszka Jaźwińska-Tarnawska, Ewa Ekk-Cierniakowski, Paweł Poniewierka, Elżbieta Wiela-Hojeńska, Anna Głowacka, Krystyna |
author_sort | Petryszyn, Paweł |
collection | PubMed |
description | P-glycoprotein encoded by the ABCB1 gene constitutes a molecular barrier in the small and large bowel epithelium, and its different expression may influence susceptibility to inflammatory bowel disease (IBD). We aimed to assess the contribution of the C3435T polymorphism to disease risk in the Polish population. A total of 100 patients (50 Crohn’s disease (CD), 50 ulcerative colitis (UC)) and 100 healthy controls were genotyped for the single nucleotide polymorphism (SNP) C3435T by using the PCR-RFLP method. Patients were classified on the basis of disease phenotype and the specific treatment used. A meta-analysis was carried out of our results and those from previously published Polish studies. There was no significant difference in allele and genotype frequencies in IBD patients compared with controls. For CD patients, a lower frequency of TT genotype in those with colonic disease, a lower frequency of T allele, and a higher frequency of C allele in those with luminal disease were observed, whereas for UC patients, a lower frequency of CT genotype was observed in those with left-sided colitis. A meta-analysis showed a tendency towards higher prevalence of CC genotype in UC cases. These results indicate that the C3435T variants may confer a risk for UC and influence disease behaviour. |
format | Online Article Text |
id | pubmed-8465101 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84651012021-09-27 C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study Petryszyn, Paweł Dudkowiak, Robert Gruca, Agnieszka Jaźwińska-Tarnawska, Ewa Ekk-Cierniakowski, Paweł Poniewierka, Elżbieta Wiela-Hojeńska, Anna Głowacka, Krystyna Genes (Basel) Article P-glycoprotein encoded by the ABCB1 gene constitutes a molecular barrier in the small and large bowel epithelium, and its different expression may influence susceptibility to inflammatory bowel disease (IBD). We aimed to assess the contribution of the C3435T polymorphism to disease risk in the Polish population. A total of 100 patients (50 Crohn’s disease (CD), 50 ulcerative colitis (UC)) and 100 healthy controls were genotyped for the single nucleotide polymorphism (SNP) C3435T by using the PCR-RFLP method. Patients were classified on the basis of disease phenotype and the specific treatment used. A meta-analysis was carried out of our results and those from previously published Polish studies. There was no significant difference in allele and genotype frequencies in IBD patients compared with controls. For CD patients, a lower frequency of TT genotype in those with colonic disease, a lower frequency of T allele, and a higher frequency of C allele in those with luminal disease were observed, whereas for UC patients, a lower frequency of CT genotype was observed in those with left-sided colitis. A meta-analysis showed a tendency towards higher prevalence of CC genotype in UC cases. These results indicate that the C3435T variants may confer a risk for UC and influence disease behaviour. MDPI 2021-09-15 /pmc/articles/PMC8465101/ /pubmed/34573401 http://dx.doi.org/10.3390/genes12091419 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Petryszyn, Paweł Dudkowiak, Robert Gruca, Agnieszka Jaźwińska-Tarnawska, Ewa Ekk-Cierniakowski, Paweł Poniewierka, Elżbieta Wiela-Hojeńska, Anna Głowacka, Krystyna C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study |
title | C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study |
title_full | C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study |
title_fullStr | C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study |
title_full_unstemmed | C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study |
title_short | C3435T Polymorphism of the ABCB1 Gene in Polish Patients with Inflammatory Bowel Disease: A Case–Control and Meta-Analysis Study |
title_sort | c3435t polymorphism of the abcb1 gene in polish patients with inflammatory bowel disease: a case–control and meta-analysis study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465101/ https://www.ncbi.nlm.nih.gov/pubmed/34573401 http://dx.doi.org/10.3390/genes12091419 |
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