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Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin
This study presents a simple route to heparin detection and develops a voltammetric approach using supramolecular principles and nanomaterials. Nanocomposites, including gold nanoparticles (AuNPs) and γ-substituted pentamethinium salts (PMS) deposited on a glass carbon (GC) electrode surface (GC/AuN...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465147/ https://www.ncbi.nlm.nih.gov/pubmed/34576581 http://dx.doi.org/10.3390/ma14185357 |
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author | Shishkanova, Tatiana V. Bříza, Tomáš Řezanka, Pavel Kejík, Zdeněk Jakubek, Milan |
author_facet | Shishkanova, Tatiana V. Bříza, Tomáš Řezanka, Pavel Kejík, Zdeněk Jakubek, Milan |
author_sort | Shishkanova, Tatiana V. |
collection | PubMed |
description | This study presents a simple route to heparin detection and develops a voltammetric approach using supramolecular principles and nanomaterials. Nanocomposites, including gold nanoparticles (AuNPs) and γ-substituted pentamethinium salts (PMS) deposited on a glass carbon (GC) electrode surface (GC/AuNPs/PMS) and covered by a plasticized poly(vinyl chloride) (PVC) membrane, are proposed for heparin detection. The conductivity of the nonconducting PVC-plasticized membrane is guaranteed by AuNPs, and the selectivity is provided by the interaction between γ-substituted PMS and anionic analytes. In order to extend the linear range, it is necessary to apply a solvent compatible with PVC-plasticized membrane, namely tetrahydrofuran. The proposed voltammetric sensor showed a concentration dependence from 1.72 up to 45.02 IU mL(−1) heparin and was used for heparin detection in saline and biological samples with recovery of 95.1–100.9%. |
format | Online Article Text |
id | pubmed-8465147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84651472021-09-27 Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin Shishkanova, Tatiana V. Bříza, Tomáš Řezanka, Pavel Kejík, Zdeněk Jakubek, Milan Materials (Basel) Article This study presents a simple route to heparin detection and develops a voltammetric approach using supramolecular principles and nanomaterials. Nanocomposites, including gold nanoparticles (AuNPs) and γ-substituted pentamethinium salts (PMS) deposited on a glass carbon (GC) electrode surface (GC/AuNPs/PMS) and covered by a plasticized poly(vinyl chloride) (PVC) membrane, are proposed for heparin detection. The conductivity of the nonconducting PVC-plasticized membrane is guaranteed by AuNPs, and the selectivity is provided by the interaction between γ-substituted PMS and anionic analytes. In order to extend the linear range, it is necessary to apply a solvent compatible with PVC-plasticized membrane, namely tetrahydrofuran. The proposed voltammetric sensor showed a concentration dependence from 1.72 up to 45.02 IU mL(−1) heparin and was used for heparin detection in saline and biological samples with recovery of 95.1–100.9%. MDPI 2021-09-17 /pmc/articles/PMC8465147/ /pubmed/34576581 http://dx.doi.org/10.3390/ma14185357 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shishkanova, Tatiana V. Bříza, Tomáš Řezanka, Pavel Kejík, Zdeněk Jakubek, Milan Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin |
title | Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin |
title_full | Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin |
title_fullStr | Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin |
title_full_unstemmed | Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin |
title_short | Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin |
title_sort | pentamethinium salts nanocomposite for electrochemical detection of heparin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465147/ https://www.ncbi.nlm.nih.gov/pubmed/34576581 http://dx.doi.org/10.3390/ma14185357 |
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