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Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches
The influenza virus is a constantly evolving pathogen that challenges medical and public health systems. Traditionally, curcumin has been used to treat airway inflammatory diseases, such as bronchitis and pneumonia. To elucidate common targets of curcumin and influenza infection and underlying mecha...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465221/ https://www.ncbi.nlm.nih.gov/pubmed/34577580 http://dx.doi.org/10.3390/ph14090880 |
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author | Kim, Minjee Choi, Hanul Kim, Sumin Kang, Lin Woo Kim, Young Bong |
author_facet | Kim, Minjee Choi, Hanul Kim, Sumin Kang, Lin Woo Kim, Young Bong |
author_sort | Kim, Minjee |
collection | PubMed |
description | The influenza virus is a constantly evolving pathogen that challenges medical and public health systems. Traditionally, curcumin has been used to treat airway inflammatory diseases, such as bronchitis and pneumonia. To elucidate common targets of curcumin and influenza infection and underlying mechanisms, we employed network pharmacology and molecular docking approaches and confirmed results using in vitro experiments. Biological targets of curcumin and influenza were collected, and potential targets were identified by constructing compound–disease target (C-D) and protein–protein interaction (PPI) networks. The ligand–target interaction was determined using the molecular docking method, and in vitro antiviral experiments and target confirmation were conducted to evaluate curcumin’s effects on influenza. Our network and pathway analyses implicated the four targets of AKT1, RELA, MAPK1, and TP53 that could be involved in the inhibitory effects of curcumin on influenza. The binding energy calculations of each ligand–target interaction in the molecular docking showed that curcumin bound to AKT1 with the highest affinity among the four targets. In vitro experiments, in which influenza virus-infected MDCK cells were pre-, co-, or post-treated with curcumin, confirmed curcumin’s prophylactic and therapeutic effects. Influenza virus induction increased the level of mRNA expression of AKT in MDCK cells, and the level was attenuated by curcumin treatment. Collectively, our findings identified potential targets of curcumin against influenza and suggest curcumin as a potential therapy for influenza infection. |
format | Online Article Text |
id | pubmed-8465221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84652212021-09-27 Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches Kim, Minjee Choi, Hanul Kim, Sumin Kang, Lin Woo Kim, Young Bong Pharmaceuticals (Basel) Article The influenza virus is a constantly evolving pathogen that challenges medical and public health systems. Traditionally, curcumin has been used to treat airway inflammatory diseases, such as bronchitis and pneumonia. To elucidate common targets of curcumin and influenza infection and underlying mechanisms, we employed network pharmacology and molecular docking approaches and confirmed results using in vitro experiments. Biological targets of curcumin and influenza were collected, and potential targets were identified by constructing compound–disease target (C-D) and protein–protein interaction (PPI) networks. The ligand–target interaction was determined using the molecular docking method, and in vitro antiviral experiments and target confirmation were conducted to evaluate curcumin’s effects on influenza. Our network and pathway analyses implicated the four targets of AKT1, RELA, MAPK1, and TP53 that could be involved in the inhibitory effects of curcumin on influenza. The binding energy calculations of each ligand–target interaction in the molecular docking showed that curcumin bound to AKT1 with the highest affinity among the four targets. In vitro experiments, in which influenza virus-infected MDCK cells were pre-, co-, or post-treated with curcumin, confirmed curcumin’s prophylactic and therapeutic effects. Influenza virus induction increased the level of mRNA expression of AKT in MDCK cells, and the level was attenuated by curcumin treatment. Collectively, our findings identified potential targets of curcumin against influenza and suggest curcumin as a potential therapy for influenza infection. MDPI 2021-08-30 /pmc/articles/PMC8465221/ /pubmed/34577580 http://dx.doi.org/10.3390/ph14090880 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Minjee Choi, Hanul Kim, Sumin Kang, Lin Woo Kim, Young Bong Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches |
title | Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches |
title_full | Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches |
title_fullStr | Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches |
title_full_unstemmed | Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches |
title_short | Elucidating the Effects of Curcumin against Influenza Using In Silico and In Vitro Approaches |
title_sort | elucidating the effects of curcumin against influenza using in silico and in vitro approaches |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465221/ https://www.ncbi.nlm.nih.gov/pubmed/34577580 http://dx.doi.org/10.3390/ph14090880 |
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