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In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta)
Rapid antigen tests (RATs) are an integral part of SARS-CoV-2 containment strategies. As emerging variants of concern (VOCs) displace the initially circulating strains, it is crucial that RATs do not fail to detect these new variants. In this study, four RATs for nasal swab testing were investigated...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465346/ https://www.ncbi.nlm.nih.gov/pubmed/34576862 http://dx.doi.org/10.3390/microorganisms9091967 |
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author | Jungnick, Sabrina Hobmaier, Bernhard Mautner, Lena Hoyos, Mona Haase, Maren Baiker, Armin Lahne, Heidi Eberle, Ute Wimmer, Clara Hepner, Sabrina Sprenger, Annika Berger, Carola Dangel, Alexandra Ippisch, Siegfried Hahner, Sonja Wildner, Manfred Liebl, Bernhard Ackermann, Nikolaus Sing, Andreas Fingerle, Volker |
author_facet | Jungnick, Sabrina Hobmaier, Bernhard Mautner, Lena Hoyos, Mona Haase, Maren Baiker, Armin Lahne, Heidi Eberle, Ute Wimmer, Clara Hepner, Sabrina Sprenger, Annika Berger, Carola Dangel, Alexandra Ippisch, Siegfried Hahner, Sonja Wildner, Manfred Liebl, Bernhard Ackermann, Nikolaus Sing, Andreas Fingerle, Volker |
author_sort | Jungnick, Sabrina |
collection | PubMed |
description | Rapid antigen tests (RATs) are an integral part of SARS-CoV-2 containment strategies. As emerging variants of concern (VOCs) displace the initially circulating strains, it is crucial that RATs do not fail to detect these new variants. In this study, four RATs for nasal swab testing were investigated using cultured strains of B.1.1 (non-VOC), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta). Based on dilution series in cell culture medium and pooled saliva, the limit of detection of these RATs was determined in a laboratory setting. Further investigations on cross-reactivity were conducted using recombinant N-protein from seasonal human coronaviruses (hCoVs). RATs evaluated showed an overall comparable performance with cultured strains of the non-VOC B.1.1 and the VOCs Alpha, Beta, Gamma, and Delta. No cross-reactivity was detected with recombinant N-protein of the hCoV strains HKU1, OC43, NL63, and 229E. A continuous evaluation of SARS-CoV-2 RAT performance is required, especially with regard to evolving mutations. Moreover, cross-reactivity and interference with pathogens and other substances on the test performance of RATs should be consistently investigated to ensure suitability in the context of SARS-CoV-2 containment. |
format | Online Article Text |
id | pubmed-8465346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84653462021-09-27 In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) Jungnick, Sabrina Hobmaier, Bernhard Mautner, Lena Hoyos, Mona Haase, Maren Baiker, Armin Lahne, Heidi Eberle, Ute Wimmer, Clara Hepner, Sabrina Sprenger, Annika Berger, Carola Dangel, Alexandra Ippisch, Siegfried Hahner, Sonja Wildner, Manfred Liebl, Bernhard Ackermann, Nikolaus Sing, Andreas Fingerle, Volker Microorganisms Communication Rapid antigen tests (RATs) are an integral part of SARS-CoV-2 containment strategies. As emerging variants of concern (VOCs) displace the initially circulating strains, it is crucial that RATs do not fail to detect these new variants. In this study, four RATs for nasal swab testing were investigated using cultured strains of B.1.1 (non-VOC), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta). Based on dilution series in cell culture medium and pooled saliva, the limit of detection of these RATs was determined in a laboratory setting. Further investigations on cross-reactivity were conducted using recombinant N-protein from seasonal human coronaviruses (hCoVs). RATs evaluated showed an overall comparable performance with cultured strains of the non-VOC B.1.1 and the VOCs Alpha, Beta, Gamma, and Delta. No cross-reactivity was detected with recombinant N-protein of the hCoV strains HKU1, OC43, NL63, and 229E. A continuous evaluation of SARS-CoV-2 RAT performance is required, especially with regard to evolving mutations. Moreover, cross-reactivity and interference with pathogens and other substances on the test performance of RATs should be consistently investigated to ensure suitability in the context of SARS-CoV-2 containment. MDPI 2021-09-16 /pmc/articles/PMC8465346/ /pubmed/34576862 http://dx.doi.org/10.3390/microorganisms9091967 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Jungnick, Sabrina Hobmaier, Bernhard Mautner, Lena Hoyos, Mona Haase, Maren Baiker, Armin Lahne, Heidi Eberle, Ute Wimmer, Clara Hepner, Sabrina Sprenger, Annika Berger, Carola Dangel, Alexandra Ippisch, Siegfried Hahner, Sonja Wildner, Manfred Liebl, Bernhard Ackermann, Nikolaus Sing, Andreas Fingerle, Volker In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) |
title | In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) |
title_full | In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) |
title_fullStr | In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) |
title_full_unstemmed | In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) |
title_short | In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta) |
title_sort | in vitro rapid antigen test performance with the sars-cov-2 variants of concern b.1.1.7 (alpha), b.1.351 (beta), p.1 (gamma), and b.1.617.2 (delta) |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465346/ https://www.ncbi.nlm.nih.gov/pubmed/34576862 http://dx.doi.org/10.3390/microorganisms9091967 |
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