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Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation

The development of biocompatible nanomaterials has become a new trend in the treatment and prevention of human amyloidosis. Human calcitonin (hCT), a hormone peptide secreted from parafollicular cells, plays a major role in calcium–phosphorus metabolism. Moreover, it can be used in the treatment of...

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Autores principales: Wu, Jhe-An, Chen, Yu-Chieh, Tu, Ling-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465381/
https://www.ncbi.nlm.nih.gov/pubmed/34578556
http://dx.doi.org/10.3390/nano11092242
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author Wu, Jhe-An
Chen, Yu-Chieh
Tu, Ling-Hsien
author_facet Wu, Jhe-An
Chen, Yu-Chieh
Tu, Ling-Hsien
author_sort Wu, Jhe-An
collection PubMed
description The development of biocompatible nanomaterials has become a new trend in the treatment and prevention of human amyloidosis. Human calcitonin (hCT), a hormone peptide secreted from parafollicular cells, plays a major role in calcium–phosphorus metabolism. Moreover, it can be used in the treatment of osteoporosis and Paget’s disease. Unfortunately, it tends to form amyloid fibrils irreversibly in an aqueous solution, resulting in a reduction of its bioavailability and therapeutic activity. Salmon calcitonin is the replacement of hCT as a widely therapeutic agent due to its lower propensity in aggregation and better bioactivity. Herein, we used citric acid to synthesize carbon dots (CDs) and modified their surface properties by a variety of chemical conjugations to provide different functionalized CDs. It was found that dopamine-conjugated CDs can effectively inhibit hCT aggregation especially in the fibril growth phase and dissociate preformed hCT amyloids. Although the decomposition mechanism of dopamine-conjugated CDs is not clear, it seems to be specific to hCT amyloids. In addition, we also tested dopamine-conjugated mesoporous silica nanoparticles in preventing hCT fibrillization. They also can work as inhibitors but are much less effective than CDs. Our studies emphasized the importance of the size and surface functionalization of core materials in the development of nanomaterials as emerging treatments for amyloidosis. On the other hand, proper functionalized CDs would be useful in hCT formulation.
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spelling pubmed-84653812021-09-27 Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation Wu, Jhe-An Chen, Yu-Chieh Tu, Ling-Hsien Nanomaterials (Basel) Article The development of biocompatible nanomaterials has become a new trend in the treatment and prevention of human amyloidosis. Human calcitonin (hCT), a hormone peptide secreted from parafollicular cells, plays a major role in calcium–phosphorus metabolism. Moreover, it can be used in the treatment of osteoporosis and Paget’s disease. Unfortunately, it tends to form amyloid fibrils irreversibly in an aqueous solution, resulting in a reduction of its bioavailability and therapeutic activity. Salmon calcitonin is the replacement of hCT as a widely therapeutic agent due to its lower propensity in aggregation and better bioactivity. Herein, we used citric acid to synthesize carbon dots (CDs) and modified their surface properties by a variety of chemical conjugations to provide different functionalized CDs. It was found that dopamine-conjugated CDs can effectively inhibit hCT aggregation especially in the fibril growth phase and dissociate preformed hCT amyloids. Although the decomposition mechanism of dopamine-conjugated CDs is not clear, it seems to be specific to hCT amyloids. In addition, we also tested dopamine-conjugated mesoporous silica nanoparticles in preventing hCT fibrillization. They also can work as inhibitors but are much less effective than CDs. Our studies emphasized the importance of the size and surface functionalization of core materials in the development of nanomaterials as emerging treatments for amyloidosis. On the other hand, proper functionalized CDs would be useful in hCT formulation. MDPI 2021-08-30 /pmc/articles/PMC8465381/ /pubmed/34578556 http://dx.doi.org/10.3390/nano11092242 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Jhe-An
Chen, Yu-Chieh
Tu, Ling-Hsien
Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation
title Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation
title_full Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation
title_fullStr Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation
title_full_unstemmed Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation
title_short Dopamine-Conjugated Carbon Dots Inhibit Human Calcitonin Fibrillation
title_sort dopamine-conjugated carbon dots inhibit human calcitonin fibrillation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465381/
https://www.ncbi.nlm.nih.gov/pubmed/34578556
http://dx.doi.org/10.3390/nano11092242
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