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Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study

Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding val...

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Autores principales: Silvestri, Giuseppe, Canedo-Ribeiro, Carla, Serrano-Albal, María, Labrecque, Remi, Blondin, Patrick, Larmer, Steven G., Marras, Gabriele, Tutt, Desmond A.R., Handyside, Alan H., Farré, Marta, Sinclair, Kevin D., Griffin, Darren K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465548/
https://www.ncbi.nlm.nih.gov/pubmed/34571932
http://dx.doi.org/10.3390/cells10092284
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author Silvestri, Giuseppe
Canedo-Ribeiro, Carla
Serrano-Albal, María
Labrecque, Remi
Blondin, Patrick
Larmer, Steven G.
Marras, Gabriele
Tutt, Desmond A.R.
Handyside, Alan H.
Farré, Marta
Sinclair, Kevin D.
Griffin, Darren K.
author_facet Silvestri, Giuseppe
Canedo-Ribeiro, Carla
Serrano-Albal, María
Labrecque, Remi
Blondin, Patrick
Larmer, Steven G.
Marras, Gabriele
Tutt, Desmond A.R.
Handyside, Alan H.
Farré, Marta
Sinclair, Kevin D.
Griffin, Darren K.
author_sort Silvestri, Giuseppe
collection PubMed
description Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p < 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p < 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes.
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spelling pubmed-84655482021-09-27 Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study Silvestri, Giuseppe Canedo-Ribeiro, Carla Serrano-Albal, María Labrecque, Remi Blondin, Patrick Larmer, Steven G. Marras, Gabriele Tutt, Desmond A.R. Handyside, Alan H. Farré, Marta Sinclair, Kevin D. Griffin, Darren K. Cells Article Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p < 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p < 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes. MDPI 2021-09-02 /pmc/articles/PMC8465548/ /pubmed/34571932 http://dx.doi.org/10.3390/cells10092284 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silvestri, Giuseppe
Canedo-Ribeiro, Carla
Serrano-Albal, María
Labrecque, Remi
Blondin, Patrick
Larmer, Steven G.
Marras, Gabriele
Tutt, Desmond A.R.
Handyside, Alan H.
Farré, Marta
Sinclair, Kevin D.
Griffin, Darren K.
Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study
title Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study
title_full Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study
title_fullStr Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study
title_full_unstemmed Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study
title_short Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study
title_sort preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: a blind retrospective study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465548/
https://www.ncbi.nlm.nih.gov/pubmed/34571932
http://dx.doi.org/10.3390/cells10092284
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