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Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease
Sarcopenia, a geriatric syndrome involving loss of muscle mass and strength, is often associated with the early phases of Alzheimer’s disease (AD). Pathological hallmarks of AD including amyloid β (Aβ) aggregates which can be found in peripheral tissues such as skeletal muscle. However, not much is...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465822/ https://www.ncbi.nlm.nih.gov/pubmed/34573265 http://dx.doi.org/10.3390/brainsci11091245 |
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author | Torcinaro, Alessio Ricci, Valentina Strimpakos, Georgios De Santa, Francesca Middei, Silvia |
author_facet | Torcinaro, Alessio Ricci, Valentina Strimpakos, Georgios De Santa, Francesca Middei, Silvia |
author_sort | Torcinaro, Alessio |
collection | PubMed |
description | Sarcopenia, a geriatric syndrome involving loss of muscle mass and strength, is often associated with the early phases of Alzheimer’s disease (AD). Pathological hallmarks of AD including amyloid β (Aβ) aggregates which can be found in peripheral tissues such as skeletal muscle. However, not much is currently known about their possible involvement in sarcopenia. We investigated neuronal innervation in skeletal muscle of Tg2576 mice, a genetic model for Aβ accumulation. We examined cholinergic innervation of skeletal muscle in adult Tg2576 and wild type mice by immunofluorescence labeling of tibialis anterior (TA) muscle sections using antibodies raised against neurofilament light chain (NFL) and acetylcholine (ACh) synthesizing enzyme choline acetyltransferase (ChAT). Combining this histological approach with real time quantification of mRNA levels of nicotinic acetylcholine receptors, we demonstrated that in the TA of Tg2576 mice, neuronal innervation is significantly reduced and synaptic area is smaller and displays less ChAT content when compared to wild type mice. Our study provides the first evidence of reduced cholinergic innervation of skeletal muscle in a mouse model of Aβ accumulation. This evidence sustains the possibility that sarcopenia in AD originates from Aβ-mediated cholinergic loss. |
format | Online Article Text |
id | pubmed-8465822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84658222021-09-27 Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease Torcinaro, Alessio Ricci, Valentina Strimpakos, Georgios De Santa, Francesca Middei, Silvia Brain Sci Article Sarcopenia, a geriatric syndrome involving loss of muscle mass and strength, is often associated with the early phases of Alzheimer’s disease (AD). Pathological hallmarks of AD including amyloid β (Aβ) aggregates which can be found in peripheral tissues such as skeletal muscle. However, not much is currently known about their possible involvement in sarcopenia. We investigated neuronal innervation in skeletal muscle of Tg2576 mice, a genetic model for Aβ accumulation. We examined cholinergic innervation of skeletal muscle in adult Tg2576 and wild type mice by immunofluorescence labeling of tibialis anterior (TA) muscle sections using antibodies raised against neurofilament light chain (NFL) and acetylcholine (ACh) synthesizing enzyme choline acetyltransferase (ChAT). Combining this histological approach with real time quantification of mRNA levels of nicotinic acetylcholine receptors, we demonstrated that in the TA of Tg2576 mice, neuronal innervation is significantly reduced and synaptic area is smaller and displays less ChAT content when compared to wild type mice. Our study provides the first evidence of reduced cholinergic innervation of skeletal muscle in a mouse model of Aβ accumulation. This evidence sustains the possibility that sarcopenia in AD originates from Aβ-mediated cholinergic loss. MDPI 2021-09-20 /pmc/articles/PMC8465822/ /pubmed/34573265 http://dx.doi.org/10.3390/brainsci11091245 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Torcinaro, Alessio Ricci, Valentina Strimpakos, Georgios De Santa, Francesca Middei, Silvia Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease |
title | Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease |
title_full | Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease |
title_fullStr | Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease |
title_full_unstemmed | Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease |
title_short | Peripheral Nerve Impairment in a Mouse Model of Alzheimer’s Disease |
title_sort | peripheral nerve impairment in a mouse model of alzheimer’s disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465822/ https://www.ncbi.nlm.nih.gov/pubmed/34573265 http://dx.doi.org/10.3390/brainsci11091245 |
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