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Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair

Endovascular repair (EVAR) has become the standard procedure in treating thoracic (TAA) or abdominal aortic aneurysms (AAA). Not entirely free of complications, a persisting perfusion of the aneurysm after EVAR, called Endoleak (EL), leads to reintervention and risk of secondary rupture. How the aor...

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Autores principales: Buerger, Matthias, Klein, Oliver, Kapahnke, Sebastian, Mueller, Verena, Frese, Jan Paul, Omran, Safwan, Greiner, Andreas, Sommerfeld, Manuela, Kaschina, Elena, Jannasch, Anett, Dittfeld, Claudia, Mahlmann, Adrian, Hinterseher, Irene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465851/
https://www.ncbi.nlm.nih.gov/pubmed/34572274
http://dx.doi.org/10.3390/biomedicines9091088
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author Buerger, Matthias
Klein, Oliver
Kapahnke, Sebastian
Mueller, Verena
Frese, Jan Paul
Omran, Safwan
Greiner, Andreas
Sommerfeld, Manuela
Kaschina, Elena
Jannasch, Anett
Dittfeld, Claudia
Mahlmann, Adrian
Hinterseher, Irene
author_facet Buerger, Matthias
Klein, Oliver
Kapahnke, Sebastian
Mueller, Verena
Frese, Jan Paul
Omran, Safwan
Greiner, Andreas
Sommerfeld, Manuela
Kaschina, Elena
Jannasch, Anett
Dittfeld, Claudia
Mahlmann, Adrian
Hinterseher, Irene
author_sort Buerger, Matthias
collection PubMed
description Endovascular repair (EVAR) has become the standard procedure in treating thoracic (TAA) or abdominal aortic aneurysms (AAA). Not entirely free of complications, a persisting perfusion of the aneurysm after EVAR, called Endoleak (EL), leads to reintervention and risk of secondary rupture. How the aortic wall responds to the implantation of a stentgraft and EL is mostly uncertain. We present a pilot study to identify peptide signatures and gain new insights in pathophysiological alterations of the aortic wall after EVAR using matrix-assisted laser desorption or ionization mass spectrometry imaging (MALDI-MSI). In course of or accompanying an open aortic repair, tissue sections from 15 patients (TAA = 5, AAA = 5, EVAR = 5) were collected. Regions of interest (tunica media and tunica adventitia) were defined and univariate (receiver operating characteristic analysis) statistical analysis for subgroup comparison was used. This proof-of-concept study demonstrates that MALDI-MSI is feasible to identify discriminatory peptide signatures separating TAA, AAA and EVAR. Decreased intensity distributions for actin, tropomyosin, and troponin after EVAR suggest impaired contractility in vascular smooth muscle cells. Furthermore, inability to provide energy caused by impaired respiratory chain function and continuous degradation of extracellular matrix components (collagen) might support aortic wall destabilization. In case of EL after EVAR, this mechanism may result in a weakened aortic wall with lacking ability to react on reinstating pulsatile blood flow.
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spelling pubmed-84658512021-09-27 Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair Buerger, Matthias Klein, Oliver Kapahnke, Sebastian Mueller, Verena Frese, Jan Paul Omran, Safwan Greiner, Andreas Sommerfeld, Manuela Kaschina, Elena Jannasch, Anett Dittfeld, Claudia Mahlmann, Adrian Hinterseher, Irene Biomedicines Article Endovascular repair (EVAR) has become the standard procedure in treating thoracic (TAA) or abdominal aortic aneurysms (AAA). Not entirely free of complications, a persisting perfusion of the aneurysm after EVAR, called Endoleak (EL), leads to reintervention and risk of secondary rupture. How the aortic wall responds to the implantation of a stentgraft and EL is mostly uncertain. We present a pilot study to identify peptide signatures and gain new insights in pathophysiological alterations of the aortic wall after EVAR using matrix-assisted laser desorption or ionization mass spectrometry imaging (MALDI-MSI). In course of or accompanying an open aortic repair, tissue sections from 15 patients (TAA = 5, AAA = 5, EVAR = 5) were collected. Regions of interest (tunica media and tunica adventitia) were defined and univariate (receiver operating characteristic analysis) statistical analysis for subgroup comparison was used. This proof-of-concept study demonstrates that MALDI-MSI is feasible to identify discriminatory peptide signatures separating TAA, AAA and EVAR. Decreased intensity distributions for actin, tropomyosin, and troponin after EVAR suggest impaired contractility in vascular smooth muscle cells. Furthermore, inability to provide energy caused by impaired respiratory chain function and continuous degradation of extracellular matrix components (collagen) might support aortic wall destabilization. In case of EL after EVAR, this mechanism may result in a weakened aortic wall with lacking ability to react on reinstating pulsatile blood flow. MDPI 2021-08-26 /pmc/articles/PMC8465851/ /pubmed/34572274 http://dx.doi.org/10.3390/biomedicines9091088 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Buerger, Matthias
Klein, Oliver
Kapahnke, Sebastian
Mueller, Verena
Frese, Jan Paul
Omran, Safwan
Greiner, Andreas
Sommerfeld, Manuela
Kaschina, Elena
Jannasch, Anett
Dittfeld, Claudia
Mahlmann, Adrian
Hinterseher, Irene
Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
title Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
title_full Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
title_fullStr Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
title_full_unstemmed Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
title_short Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
title_sort use of maldi mass spectrometry imaging to identify proteomic signatures in aortic aneurysms after endovascular repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465851/
https://www.ncbi.nlm.nih.gov/pubmed/34572274
http://dx.doi.org/10.3390/biomedicines9091088
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