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Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS

MALDI TOF MS-based microbial identification significantly lowers the operational costs because of minimal requirements of substrates and reagents for extraction. Therefore, it has been widely used in varied applications such as clinical, food, military, and ecological research. However, the MALDI TO...

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Autores principales: Han, Sang-Soo, Jeong, Young-Su, Choi, Sun-Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466008/
https://www.ncbi.nlm.nih.gov/pubmed/34576812
http://dx.doi.org/10.3390/microorganisms9091917
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author Han, Sang-Soo
Jeong, Young-Su
Choi, Sun-Kyung
author_facet Han, Sang-Soo
Jeong, Young-Su
Choi, Sun-Kyung
author_sort Han, Sang-Soo
collection PubMed
description MALDI TOF MS-based microbial identification significantly lowers the operational costs because of minimal requirements of substrates and reagents for extraction. Therefore, it has been widely used in varied applications such as clinical, food, military, and ecological research. However, the MALDI TOF MS method is laced with many challenges including its limitation of the reference spectrum. This review briefly introduces the background of MALDI TOF MS technology, including sample preparation and workflow. We have primarily discussed the application of MALDI TOF MS in the identification of microorganisms. Furthermore, we have discussed the current trends for bioaerosol detection using MALDI TOF MS and the limitations and challenges involved, and finally the approaches to overcome these challenges.
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spelling pubmed-84660082021-09-27 Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS Han, Sang-Soo Jeong, Young-Su Choi, Sun-Kyung Microorganisms Review MALDI TOF MS-based microbial identification significantly lowers the operational costs because of minimal requirements of substrates and reagents for extraction. Therefore, it has been widely used in varied applications such as clinical, food, military, and ecological research. However, the MALDI TOF MS method is laced with many challenges including its limitation of the reference spectrum. This review briefly introduces the background of MALDI TOF MS technology, including sample preparation and workflow. We have primarily discussed the application of MALDI TOF MS in the identification of microorganisms. Furthermore, we have discussed the current trends for bioaerosol detection using MALDI TOF MS and the limitations and challenges involved, and finally the approaches to overcome these challenges. MDPI 2021-09-09 /pmc/articles/PMC8466008/ /pubmed/34576812 http://dx.doi.org/10.3390/microorganisms9091917 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Han, Sang-Soo
Jeong, Young-Su
Choi, Sun-Kyung
Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS
title Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS
title_full Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS
title_fullStr Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS
title_full_unstemmed Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS
title_short Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS
title_sort current scenario and challenges in the direct identification of microorganisms using maldi tof ms
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466008/
https://www.ncbi.nlm.nih.gov/pubmed/34576812
http://dx.doi.org/10.3390/microorganisms9091917
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