Neurohormonal Profiles of Assistance Dogs Compared to Pet Dogs: What Is the Impact of Different Lifestyles?

SIMPLE SUMMARY: Dogs are currently involved in various roles in our society beyond companionship. The tasks humans assign to them impact their daily life and can sometimes create stressful situations, possibly jeopardizing their welfare. For example, assistance dogs need to manage their emotions in various challenging situations and environments. Thus, the capacity to cope with emotional stress is highly desirable in assistance dogs (~40% of assistance dogs fail to complete their education program). The emotional and stress responses are guided by brain processes involving neuromodulators. Neurohormonal profiling of these dogs can: (i) give cues about their emotional suitability to fulfill an assistance role; (ii) enhance their selection; and (iii) help to assess and improve their welfare state during the training course. We compared basal blood levels of three neuromodulators of interest between two populations, assistance vs. pet dogs. We found significantly different concentrations of oxytocin, a neuromodulator involved in social behavior. Levels of prolactin, a putative marker of chronic stress, were higher (although not statistically significant) and variable in assistance dogs. Dogs’ age also seemed to influence the various neuromodulators levels. These findings highlight the impact of different lifestyles undergone by dogs and the possibility to use neurohormonal profiling to monitor their effect on the dogs’ welfare and stress state. ABSTRACT: Assistance dogs must manage stress efficiently because they are involved in challenging tasks. Their welfare is currently a fundamental issue. This preliminary study aimed to compare assistance dogs (AD; n = 22) with pet dogs (PD; n = 24), using blood neuromodulator indicators to help find biomarkers that can improve the AD breeding, selection, training, and welfare monitoring. Both populations originated from different breeds, are of different ages, and had different lifestyles. Basal peripheral concentrations of prolactin (PRL), serotonin (5-HT), free (fOT) and total (tOT) oxytocin were measured by immunoassays. Multiple linear regressions were performed to assess the effect of activity, age, sex, and their interactions on these parameters. Correlations between neurohormonal levels were analyzed. No interactions were significant. fOT and tOT concentrations were significantly influenced by age (p < 0.0001 and p = 0.0002, respectively) and dogs’ activity (p = 0.0006 and p = 0.0277, respectively). A tendency was observed for age effect on PRL (p = 0.0625) and 5-HT (p = 0.0548), as well as for sex effect on tOT (p = 0.0588). PRL concentrations were heterogenous among AD. fOT and tOT were significantly but weakly correlated (Pearson’s r = 0.34; p = 0.04). Blood prolactin, serotonin, and oxytocin may represent biomarkers to assess workload and chronic stress-related responses in ADs and eventually improve their selection and training.