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Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH

SIMPLE SUMMARY: The pituitary gland, an important endocrine organ, can secrete a variety of reproductive hormones under the action of hypothalamus-secreted gonadotropin-releasing hormone. Circular RNAs are a class of RNA molecules with stable covalently closed circular structures. In this study, we...

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Autores principales: Guo, Hai-Xiang, Yuan, Bao, Su, Meng-Ting, Zheng, Yi, Zhang, Jin-Yu, Han, Dong-Xu, Wang, Hao-Qi, Huang, Yi-Jie, Jiang, Hao, Zhang, Jia-Bao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466137/
https://www.ncbi.nlm.nih.gov/pubmed/34573523
http://dx.doi.org/10.3390/ani11092557
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author Guo, Hai-Xiang
Yuan, Bao
Su, Meng-Ting
Zheng, Yi
Zhang, Jin-Yu
Han, Dong-Xu
Wang, Hao-Qi
Huang, Yi-Jie
Jiang, Hao
Zhang, Jia-Bao
author_facet Guo, Hai-Xiang
Yuan, Bao
Su, Meng-Ting
Zheng, Yi
Zhang, Jin-Yu
Han, Dong-Xu
Wang, Hao-Qi
Huang, Yi-Jie
Jiang, Hao
Zhang, Jia-Bao
author_sort Guo, Hai-Xiang
collection PubMed
description SIMPLE SUMMARY: The pituitary gland, an important endocrine organ, can secrete a variety of reproductive hormones under the action of hypothalamus-secreted gonadotropin-releasing hormone. Circular RNAs are a class of RNA molecules with stable covalently closed circular structures. In this study, we performed RNA sequencing of GnRH-treated rats to identify differentially expressed circRNAs in the anterior pituitary. The results revealed 1433 related circRNAs, 14 of which were differentially expressed. We predicted targeted relationships between the differentially expressed circRNAs and FSHb-LHb-associated miRNAs. In all, a total of 14 circRNAs were identified that may act on the secretion and regulation of reproductive hormones in GnRH-treated rats. ABSTRACT: The pituitary gland, an important endocrine organ, can secrete a variety of reproductive hormones under the action of hypothalamus-secreted gonadotropin-releasing hormone (GnRH) and plays important roles in animal reproduction. Circular RNAs (circRNAs) are a class of RNA molecules with stable covalently closed circular structures. CircRNAs are equipped with miRNA response elements (MREs), which can regulate the expression of target genes by competitively binding miRNAs. However, whether the expression levels of circRNAs in the pituitary gland change under the action of GnRH and whether such changes can further affect the secretion of reproductive hormones are still unclear. In this study, we performed RNA sequencing (RNA-Seq) of GnRH-treated rats to identify differentially expressed circRNAs. The results revealed 1433 related circRNAs, 14 of which were differentially expressed. In addition, we randomly selected five differentially expressed circRNAs and tested their relative expression levels by RT-qPCR, the results of which were consistent with the RNA sequencing results. Finally, we predicted targeted relationships between the differentially expressed circRNAs and FSHb-LHb-associated miRNAs. In all, a total of 14 circRNAs were identified that may act on the secretion and regulation of reproductive hormones in GnRH-treated rats. Our expression profiles of circRNAs in the anterior pituitaries of rats treated with GnRH can provide insights into the roles of circRNAs in mammalian development and reproduction.
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spelling pubmed-84661372021-09-27 Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH Guo, Hai-Xiang Yuan, Bao Su, Meng-Ting Zheng, Yi Zhang, Jin-Yu Han, Dong-Xu Wang, Hao-Qi Huang, Yi-Jie Jiang, Hao Zhang, Jia-Bao Animals (Basel) Article SIMPLE SUMMARY: The pituitary gland, an important endocrine organ, can secrete a variety of reproductive hormones under the action of hypothalamus-secreted gonadotropin-releasing hormone. Circular RNAs are a class of RNA molecules with stable covalently closed circular structures. In this study, we performed RNA sequencing of GnRH-treated rats to identify differentially expressed circRNAs in the anterior pituitary. The results revealed 1433 related circRNAs, 14 of which were differentially expressed. We predicted targeted relationships between the differentially expressed circRNAs and FSHb-LHb-associated miRNAs. In all, a total of 14 circRNAs were identified that may act on the secretion and regulation of reproductive hormones in GnRH-treated rats. ABSTRACT: The pituitary gland, an important endocrine organ, can secrete a variety of reproductive hormones under the action of hypothalamus-secreted gonadotropin-releasing hormone (GnRH) and plays important roles in animal reproduction. Circular RNAs (circRNAs) are a class of RNA molecules with stable covalently closed circular structures. CircRNAs are equipped with miRNA response elements (MREs), which can regulate the expression of target genes by competitively binding miRNAs. However, whether the expression levels of circRNAs in the pituitary gland change under the action of GnRH and whether such changes can further affect the secretion of reproductive hormones are still unclear. In this study, we performed RNA sequencing (RNA-Seq) of GnRH-treated rats to identify differentially expressed circRNAs. The results revealed 1433 related circRNAs, 14 of which were differentially expressed. In addition, we randomly selected five differentially expressed circRNAs and tested their relative expression levels by RT-qPCR, the results of which were consistent with the RNA sequencing results. Finally, we predicted targeted relationships between the differentially expressed circRNAs and FSHb-LHb-associated miRNAs. In all, a total of 14 circRNAs were identified that may act on the secretion and regulation of reproductive hormones in GnRH-treated rats. Our expression profiles of circRNAs in the anterior pituitaries of rats treated with GnRH can provide insights into the roles of circRNAs in mammalian development and reproduction. MDPI 2021-08-31 /pmc/articles/PMC8466137/ /pubmed/34573523 http://dx.doi.org/10.3390/ani11092557 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Hai-Xiang
Yuan, Bao
Su, Meng-Ting
Zheng, Yi
Zhang, Jin-Yu
Han, Dong-Xu
Wang, Hao-Qi
Huang, Yi-Jie
Jiang, Hao
Zhang, Jia-Bao
Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH
title Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH
title_full Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH
title_fullStr Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH
title_full_unstemmed Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH
title_short Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH
title_sort identification of circular rnas in the anterior pituitary in rats treated with gnrh
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466137/
https://www.ncbi.nlm.nih.gov/pubmed/34573523
http://dx.doi.org/10.3390/ani11092557
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