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Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach
Acellular amniotic membrane (AM) has been studied, with promising results on the reconstruction of lesioned tissues, and has become an attractive approach for tracheal repair. This study aimed to evaluate the repair of the trachea with human umbilical cord mesenchymal stem cells (hucMSCs) differenti...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466253/ https://www.ncbi.nlm.nih.gov/pubmed/34575028 http://dx.doi.org/10.3390/life11090879 |
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author | Baggio Simeoni, Paulo Ricardo Simeoni, Rossana Baggio Bispo Machado Júnior, Paulo André de Almeida, Meila Bastos Dziedzic, Dilcele Silva Moreira da Rosa, Nádia Nascimento Ferreira Stricker, Priscila E. dos Santos Miggiolaro, Anna Flávia Ribeiro Naves, Guilherme Neto, Nelson Bergonse de Noronha, Lucia Francisco, Julio Cesar Teixeira de Carvalho, Katherine Athayde Guarita-Souza, Luiz Cesar |
author_facet | Baggio Simeoni, Paulo Ricardo Simeoni, Rossana Baggio Bispo Machado Júnior, Paulo André de Almeida, Meila Bastos Dziedzic, Dilcele Silva Moreira da Rosa, Nádia Nascimento Ferreira Stricker, Priscila E. dos Santos Miggiolaro, Anna Flávia Ribeiro Naves, Guilherme Neto, Nelson Bergonse de Noronha, Lucia Francisco, Julio Cesar Teixeira de Carvalho, Katherine Athayde Guarita-Souza, Luiz Cesar |
author_sort | Baggio Simeoni, Paulo Ricardo |
collection | PubMed |
description | Acellular amniotic membrane (AM) has been studied, with promising results on the reconstruction of lesioned tissues, and has become an attractive approach for tracheal repair. This study aimed to evaluate the repair of the trachea with human umbilical cord mesenchymal stem cells (hucMSCs) differentiated in chondrocytes, grown on an experimental model. Tracheal defects were induced by surgical tracheostomy in 30 New Zealand rabbits, and the acellular amniotic membrane, with or without cells, was covering the defect. The hucMSCs were isolated and cultivated with chondrogenic differentiation over the culture of 14 days, and then grown on the AM. In this study, the AM was biocompatible and hucMSCs differentiated into chondrocytes. Our results demonstrated an important role for AM with cultured cells in the promotion of immature collagen, known to produce tissue regeneration. In addition, cartilaginous tissue was found at the tracheal defects, demonstrated by immunohistology results. This study suggests that this biomaterial implantation can be an effective future therapeutic alternative for patients with tracheal injury. |
format | Online Article Text |
id | pubmed-8466253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84662532021-09-27 Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach Baggio Simeoni, Paulo Ricardo Simeoni, Rossana Baggio Bispo Machado Júnior, Paulo André de Almeida, Meila Bastos Dziedzic, Dilcele Silva Moreira da Rosa, Nádia Nascimento Ferreira Stricker, Priscila E. dos Santos Miggiolaro, Anna Flávia Ribeiro Naves, Guilherme Neto, Nelson Bergonse de Noronha, Lucia Francisco, Julio Cesar Teixeira de Carvalho, Katherine Athayde Guarita-Souza, Luiz Cesar Life (Basel) Article Acellular amniotic membrane (AM) has been studied, with promising results on the reconstruction of lesioned tissues, and has become an attractive approach for tracheal repair. This study aimed to evaluate the repair of the trachea with human umbilical cord mesenchymal stem cells (hucMSCs) differentiated in chondrocytes, grown on an experimental model. Tracheal defects were induced by surgical tracheostomy in 30 New Zealand rabbits, and the acellular amniotic membrane, with or without cells, was covering the defect. The hucMSCs were isolated and cultivated with chondrogenic differentiation over the culture of 14 days, and then grown on the AM. In this study, the AM was biocompatible and hucMSCs differentiated into chondrocytes. Our results demonstrated an important role for AM with cultured cells in the promotion of immature collagen, known to produce tissue regeneration. In addition, cartilaginous tissue was found at the tracheal defects, demonstrated by immunohistology results. This study suggests that this biomaterial implantation can be an effective future therapeutic alternative for patients with tracheal injury. MDPI 2021-08-26 /pmc/articles/PMC8466253/ /pubmed/34575028 http://dx.doi.org/10.3390/life11090879 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baggio Simeoni, Paulo Ricardo Simeoni, Rossana Baggio Bispo Machado Júnior, Paulo André de Almeida, Meila Bastos Dziedzic, Dilcele Silva Moreira da Rosa, Nádia Nascimento Ferreira Stricker, Priscila E. dos Santos Miggiolaro, Anna Flávia Ribeiro Naves, Guilherme Neto, Nelson Bergonse de Noronha, Lucia Francisco, Julio Cesar Teixeira de Carvalho, Katherine Athayde Guarita-Souza, Luiz Cesar Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach |
title | Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach |
title_full | Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach |
title_fullStr | Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach |
title_full_unstemmed | Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach |
title_short | Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach |
title_sort | tracheal repair with human umbilical cord mesenchymal stem cells differentiated in chondrocytes grown on an acellular amniotic membrane: a pre-clinical approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466253/ https://www.ncbi.nlm.nih.gov/pubmed/34575028 http://dx.doi.org/10.3390/life11090879 |
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