Cargando…

Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line...

Descripción completa

Detalles Bibliográficos
Autores principales:	Olenginski, Lukasz T., Taiwo, Kehinde M., LeBlanc, Regan M., Dayie, Theodore K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Review
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466439/ https://www.ncbi.nlm.nih.gov/pubmed/34577051 http://dx.doi.org/10.3390/molecules26185581

Ejemplares similares

Isotope Labels Combined with Solution NMR Spectroscopy Make Visible the Invisible Conformations of Small-to-Large RNAs
por: Dayie, Theodore K., et al.
Publicado: (2022)

Solid-Phase Chemical Synthesis of Stable Isotope-Labeled RNA to Aid Structure and Dynamics Studies by NMR Spectroscopy
por: Becette, Owen, et al.
Publicado: (2019)

Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy
por: Taiwo, Kehinde M., et al.
Publicado: (2022)

Hepatitis B Virus Epsilon (ε) RNA Element: Dynamic Regulator of Viral Replication and Attractive Therapeutic Target
por: Olenginski, Lukasz T., et al.
Publicado: (2023)

Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations
por: Longhini, Andrew P., et al.
Publicado: (2016)

Combining asymmetric (13)C-labeling and isotopic filter/edit NOESY: a novel strategy for rapid and logical RNA resonance assignment
por: LeBlanc, Regan M., et al.
Publicado: (2017)

Quantifying the effects of long-range (13)C-(13)C dipolar coupling on measured relaxation rates in RNA
por: Olenginski, Lukasz T., et al.
Publicado: (2021)

Solution NMR readily reveals distinct structural folds and interactions in doubly (13)C- and (19)F-labeled RNAs
por: Becette, Owen B., et al.
Publicado: (2020)

Isotope labeling strategies for NMR studies of RNA
por: Lu, Kun, et al.
Publicado: (2009)

Virtual Screening of Hepatitis B Virus Pre-Genomic RNA as a Novel Therapeutic Target
por: Olenginski, Lukasz T., et al.
Publicado: (2023)

Enzymatic incorporation of an isotope-labeled adenine into RNA for the study of conformational dynamics by NMR
por: Feyrer, Hannes, et al.
Publicado: (2022)

Asymmetry of (13)C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy
por: Thakur, Chandar S., et al.
Publicado: (2011)

Asymmetry of (13)C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy
por: Thakur, Chandar S., et al.
Publicado: (2011)

Synthesis and incorporation of (13)C-labeled DNA building blocks to probe structural dynamics of DNA by NMR
por: Nußbaumer, Felix, et al.
Publicado: (2017)

Multiple segmental and selective isotope labeling of large RNA for NMR structural studies
por: Nelissen, Frank H. T., et al.
Publicado: (2008)

Site-specific labeling of nucleotides for making RNA for high resolution NMR studies using an E. coli strain disabled in the oxidative pentose phosphate pathway
por: Dayie, T. Kwaku, et al.
Publicado: (2010)

Selective (13)C labeling of nucleotides for large RNA NMR spectroscopy using an E. coli strain disabled in the TCA cycle
por: Thakur, Chandar S., et al.
Publicado: (2010)

Synthesis of Isotopically Labeled, Spin-Isolated Tyrosine and Phenylalanine for Protein NMR Applications
por: Young, Brandon M., et al.
Publicado: (2021)

Selective isotope labeling for NMR structure determination of proteins in complex with unlabeled ligands
por: Tripsianes, Konstantinos, et al.
Publicado: (2019)

(82)Se Metabolically-Labeled Yeast as a Matrix-Matched Isotope Dilution Standard for Quantification of Selenomethionine
por: LeBlanc, Kelly L., et al.
Publicado: (2023)

Isotopic evolution of the protoplanetary disk and the building blocks of Earth and Moon
por: Schiller, Martin, et al.
Publicado: (2018)

Specific isotopic labelling and reverse labelling for protein NMR spectroscopy: using metabolic precursors in sample preparation
por: Rowlinson, Benjamin, et al.
Publicado: (2022)

Key Labeling Technologies to Tackle Sizeable Problems in RNA Structural Biology
por: Dayie, Kwaku T.
Publicado: (2008)

Phosphoramidite building blocks with protected nitroxides for the synthesis of spin-labeled DNA and RNA
por: Weinrich, Timo, et al.
Publicado: (2018)

NMR Structure and Dynamics of TonB Investigated by Scar-Less Segmental Isotopic Labeling Using a Salt-Inducible Split Intein
por: Ciragan, Annika, et al.
Publicado: (2020)

The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins
por: Schweida, David, et al.
Publicado: (2019)

Biomass production of site selective (13)C/(15)N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR
por: Thakur, Chandar S., et al.
Publicado: (2011)

Simulation of the NMR response in the pseudogap regime of the cuprates
por: Chen, Xi, et al.
Publicado: (2017)

Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions
por: Vesely, Cat Hoang, et al.
Publicado: (2022)

Chemical Analysis of an Isotopically Labeled Molecule Using Two-Dimensional NMR Spectroscopy at 34 μT
por: Lee, Seong-Joo, et al.
Publicado: (2023)

Extending the toolbox for RNA biology with SegModTeX: A polymerase-driven method for site-specific and segmental labeling of RNA
por: Haslecker, Raphael, et al.
Publicado: (2023)

Building blocks for automated elucidation of metabolites: Machine learning methods for NMR prediction
por: Kuhn, Stefan, et al.
Publicado: (2008)

Stable-Isotope-Aided NMR Spectroscopy
por: Miyanoiri, Yohei, et al.
Publicado: (2018)

A magnesium-induced triplex pre-organizes the SAM-II riboswitch
por: Roy, Susmita, et al.
Publicado: (2017)

Solid-state (17)O NMR study of α-d-glucose: exploring new frontiers in isotopic labeling, sensitivity enhancement, and NMR crystallography
por: Shen, Jiahui, et al.
Publicado: (2022)

Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling
por: Liu, Dongsheng, et al.
Publicado: (2016)

The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy
por: Opdensteinen, Patrick, et al.
Publicado: (2022)

Biocatalytic reductive amination as a route to isotopically labelled amino acids suitable for analysis of large proteins by NMR
por: Rowbotham, Jack S., et al.
Publicado: (2023)

Early formation of planetary building blocks inferred from Pb isotopic ages of chondrules
por: Bollard, Jean, et al.
Publicado: (2017)

$\beta$-NMR of copper isotopes in ionic liquids
por: Stachura, Monika
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_e34manc6aj12ichvipgjfdu010