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Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells

The anionic cobaltabis (dicarbollide) [3,3′-Co(1,2-C(2)B(9)H(11))(2)](−), [o-COSAN](−), is the most studied icosahedral metallacarborane. The sodium salts of [o-COSAN](−) could be an ideal candidate for the anti-cancer treatment Boron Neutron Capture Therapy (BNCT) as it possesses the ability to rea...

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Autores principales: Nuez-Martínez, Miquel, Pedrosa, Leire, Martinez-Rovira, Immaculada, Yousef, Ibraheem, Diao, Diouldé, Teixidor, Francesc, Stanzani, Elisabetta, Martínez-Soler, Fina, Tortosa, Avelina, Sierra, Àngels, Gonzalez, José Juan, Viñas, Clara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466526/
https://www.ncbi.nlm.nih.gov/pubmed/34576098
http://dx.doi.org/10.3390/ijms22189937
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author Nuez-Martínez, Miquel
Pedrosa, Leire
Martinez-Rovira, Immaculada
Yousef, Ibraheem
Diao, Diouldé
Teixidor, Francesc
Stanzani, Elisabetta
Martínez-Soler, Fina
Tortosa, Avelina
Sierra, Àngels
Gonzalez, José Juan
Viñas, Clara
author_facet Nuez-Martínez, Miquel
Pedrosa, Leire
Martinez-Rovira, Immaculada
Yousef, Ibraheem
Diao, Diouldé
Teixidor, Francesc
Stanzani, Elisabetta
Martínez-Soler, Fina
Tortosa, Avelina
Sierra, Àngels
Gonzalez, José Juan
Viñas, Clara
author_sort Nuez-Martínez, Miquel
collection PubMed
description The anionic cobaltabis (dicarbollide) [3,3′-Co(1,2-C(2)B(9)H(11))(2)](−), [o-COSAN](−), is the most studied icosahedral metallacarborane. The sodium salts of [o-COSAN](−) could be an ideal candidate for the anti-cancer treatment Boron Neutron Capture Therapy (BNCT) as it possesses the ability to readily cross biological membranes thereby producing cell cycle arrest in cancer cells. BNCT is a cancer therapy based on the potential of (10)B atoms to produce α particles that cross tissues in which the (10)B is accumulated without damaging the surrounding healthy tissues, after being irradiated with low energy thermal neutrons. Since Na[o-COSAN] displays a strong and characteristic ν(B-H) frequency in the infrared range 2.600–2.500 cm(−1), we studied the uptake of Na[o-COSAN] followed by its interaction with biomolecules and its cellular biodistribution in two different glioma initiating cells (GICs), mesenchymal and proneural respectively, by using Synchrotron Radiation-Fourier Transform Infrared (FTIR) micro-spectroscopy (SR-FTIRM) facilities at the MIRAS Beamline of ALBA synchrotron light source. The spectroscopic data analysis from the bands in the regions of DNA, proteins, and lipids permitted to suggest that after its cellular uptake, Na[o-COSAN] strongly interacts with DNA strings, modifies proteins secondary structure and also leads to lipid saturation. The mapping suggests the nuclear localization of [o-COSAN](−), which according to reported Monte Carlo simulations may result in a more efficient cell-killing effect compared to that in a uniform distribution within the entire cell. In conclusion, we show pieces of evidence that at low doses, [o-COSAN](−) translocates GIC cells’ membranes and it alters the physiology of the cells, suggesting that Na[o-COSAN] is a promising agent to BNCT for glioblastoma cells.
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spelling pubmed-84665262021-09-27 Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells Nuez-Martínez, Miquel Pedrosa, Leire Martinez-Rovira, Immaculada Yousef, Ibraheem Diao, Diouldé Teixidor, Francesc Stanzani, Elisabetta Martínez-Soler, Fina Tortosa, Avelina Sierra, Àngels Gonzalez, José Juan Viñas, Clara Int J Mol Sci Article The anionic cobaltabis (dicarbollide) [3,3′-Co(1,2-C(2)B(9)H(11))(2)](−), [o-COSAN](−), is the most studied icosahedral metallacarborane. The sodium salts of [o-COSAN](−) could be an ideal candidate for the anti-cancer treatment Boron Neutron Capture Therapy (BNCT) as it possesses the ability to readily cross biological membranes thereby producing cell cycle arrest in cancer cells. BNCT is a cancer therapy based on the potential of (10)B atoms to produce α particles that cross tissues in which the (10)B is accumulated without damaging the surrounding healthy tissues, after being irradiated with low energy thermal neutrons. Since Na[o-COSAN] displays a strong and characteristic ν(B-H) frequency in the infrared range 2.600–2.500 cm(−1), we studied the uptake of Na[o-COSAN] followed by its interaction with biomolecules and its cellular biodistribution in two different glioma initiating cells (GICs), mesenchymal and proneural respectively, by using Synchrotron Radiation-Fourier Transform Infrared (FTIR) micro-spectroscopy (SR-FTIRM) facilities at the MIRAS Beamline of ALBA synchrotron light source. The spectroscopic data analysis from the bands in the regions of DNA, proteins, and lipids permitted to suggest that after its cellular uptake, Na[o-COSAN] strongly interacts with DNA strings, modifies proteins secondary structure and also leads to lipid saturation. The mapping suggests the nuclear localization of [o-COSAN](−), which according to reported Monte Carlo simulations may result in a more efficient cell-killing effect compared to that in a uniform distribution within the entire cell. In conclusion, we show pieces of evidence that at low doses, [o-COSAN](−) translocates GIC cells’ membranes and it alters the physiology of the cells, suggesting that Na[o-COSAN] is a promising agent to BNCT for glioblastoma cells. MDPI 2021-09-14 /pmc/articles/PMC8466526/ /pubmed/34576098 http://dx.doi.org/10.3390/ijms22189937 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nuez-Martínez, Miquel
Pedrosa, Leire
Martinez-Rovira, Immaculada
Yousef, Ibraheem
Diao, Diouldé
Teixidor, Francesc
Stanzani, Elisabetta
Martínez-Soler, Fina
Tortosa, Avelina
Sierra, Àngels
Gonzalez, José Juan
Viñas, Clara
Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells
title Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells
title_full Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells
title_fullStr Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells
title_full_unstemmed Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells
title_short Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells
title_sort synchrotron-based fourier-transform infrared micro-spectroscopy (sr-ftirm) fingerprint of the small anionic molecule cobaltabis(dicarbollide) uptake in glioma stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466526/
https://www.ncbi.nlm.nih.gov/pubmed/34576098
http://dx.doi.org/10.3390/ijms22189937
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