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The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation

Background: Growth factors and cytokines responsible for the regenerative potential of the dental pulp mesenchymal stem cell secretome (DPMSC-S) are implicated in oral carcinogenesis. The impact and effects of these secretory factors on cancer cells must be understood in order to ensure their safe a...

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Autores principales: Raj, A. Thirumal, Kheur, Supriya, Khurshid, Zohaib, Sayed, Mohammed E., Mugri, Maryam H., Almasri, Mazen A., Al-Ahmari, Manea Musa, Patil, Vikrant R., Bhandi, Shilpa, Testarelli, Luca, Patil, Shankargouda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466568/
https://www.ncbi.nlm.nih.gov/pubmed/34577154
http://dx.doi.org/10.3390/molecules26185683
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author Raj, A. Thirumal
Kheur, Supriya
Khurshid, Zohaib
Sayed, Mohammed E.
Mugri, Maryam H.
Almasri, Mazen A.
Al-Ahmari, Manea Musa
Patil, Vikrant R.
Bhandi, Shilpa
Testarelli, Luca
Patil, Shankargouda
author_facet Raj, A. Thirumal
Kheur, Supriya
Khurshid, Zohaib
Sayed, Mohammed E.
Mugri, Maryam H.
Almasri, Mazen A.
Al-Ahmari, Manea Musa
Patil, Vikrant R.
Bhandi, Shilpa
Testarelli, Luca
Patil, Shankargouda
author_sort Raj, A. Thirumal
collection PubMed
description Background: Growth factors and cytokines responsible for the regenerative potential of the dental pulp mesenchymal stem cell secretome (DPMSC-S) are implicated in oral carcinogenesis. The impact and effects of these secretory factors on cancer cells must be understood in order to ensure their safe application in cancer patients. Objective: We aimed to quantify the growth factors and cytokines in DPMSC-S and assess their effect on oral cancer cell proliferation. Materials and methods: DPMSCs were isolated from patients with healthy teeth (n = 5) that were indicated for extraction for orthodontic reasons. The cells were characterized using flow cytometry and conditioned medium (DPMSC-CM) was prepared. DPMSC-CM was subjected to a bead-based array to quantify the growth factors and cytokines that may affect oral carcinogenesis. The effect of DPMSC-CM (20%, 50%, 100%) on the proliferation of oral cancer cells (AW123516) was evaluated using a Ki-67-based assay at 48 h. AW13516 cultured in the standard growth medium acted as the control. Results: VEGF, HCF, Ang-2, TGF-α, EPO, SCF, FGF, and PDGF-BB were the growth factors with the highest levels in the DPMSC-CM. The highest measured pro-inflammatory cytokine was TNF-α, followed by CXCL8. The most prevalent anti-inflammatory cytokine in the DPMSC-CM was IL-10, followed by TGF-β1 and IL-4. Concentrations of 50% and 100% DPMSC-CM inhibited Ki-67 expression in AW13516, although the effect was non-significant. Moreover, 20% DPMSC-CM significantly increased Ki-67 expression compared to the control. Conclusions: The increased Ki-67 expression of oral cancer cells in response to 20% DPMSC-CM indicates the potential for cancer progression. Further research is needed to identify their effects on other carcinogenic properties, including apoptosis, stemness, migration, invasion, adhesion, and therapeutic resistance.
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spelling pubmed-84665682021-09-27 The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation Raj, A. Thirumal Kheur, Supriya Khurshid, Zohaib Sayed, Mohammed E. Mugri, Maryam H. Almasri, Mazen A. Al-Ahmari, Manea Musa Patil, Vikrant R. Bhandi, Shilpa Testarelli, Luca Patil, Shankargouda Molecules Article Background: Growth factors and cytokines responsible for the regenerative potential of the dental pulp mesenchymal stem cell secretome (DPMSC-S) are implicated in oral carcinogenesis. The impact and effects of these secretory factors on cancer cells must be understood in order to ensure their safe application in cancer patients. Objective: We aimed to quantify the growth factors and cytokines in DPMSC-S and assess their effect on oral cancer cell proliferation. Materials and methods: DPMSCs were isolated from patients with healthy teeth (n = 5) that were indicated for extraction for orthodontic reasons. The cells were characterized using flow cytometry and conditioned medium (DPMSC-CM) was prepared. DPMSC-CM was subjected to a bead-based array to quantify the growth factors and cytokines that may affect oral carcinogenesis. The effect of DPMSC-CM (20%, 50%, 100%) on the proliferation of oral cancer cells (AW123516) was evaluated using a Ki-67-based assay at 48 h. AW13516 cultured in the standard growth medium acted as the control. Results: VEGF, HCF, Ang-2, TGF-α, EPO, SCF, FGF, and PDGF-BB were the growth factors with the highest levels in the DPMSC-CM. The highest measured pro-inflammatory cytokine was TNF-α, followed by CXCL8. The most prevalent anti-inflammatory cytokine in the DPMSC-CM was IL-10, followed by TGF-β1 and IL-4. Concentrations of 50% and 100% DPMSC-CM inhibited Ki-67 expression in AW13516, although the effect was non-significant. Moreover, 20% DPMSC-CM significantly increased Ki-67 expression compared to the control. Conclusions: The increased Ki-67 expression of oral cancer cells in response to 20% DPMSC-CM indicates the potential for cancer progression. Further research is needed to identify their effects on other carcinogenic properties, including apoptosis, stemness, migration, invasion, adhesion, and therapeutic resistance. MDPI 2021-09-19 /pmc/articles/PMC8466568/ /pubmed/34577154 http://dx.doi.org/10.3390/molecules26185683 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raj, A. Thirumal
Kheur, Supriya
Khurshid, Zohaib
Sayed, Mohammed E.
Mugri, Maryam H.
Almasri, Mazen A.
Al-Ahmari, Manea Musa
Patil, Vikrant R.
Bhandi, Shilpa
Testarelli, Luca
Patil, Shankargouda
The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation
title The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation
title_full The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation
title_fullStr The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation
title_full_unstemmed The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation
title_short The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation
title_sort growth factors and cytokines of dental pulp mesenchymal stem cell secretome may potentially aid in oral cancer proliferation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466568/
https://www.ncbi.nlm.nih.gov/pubmed/34577154
http://dx.doi.org/10.3390/molecules26185683
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