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LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model
Cationic anticancer peptides have exhibited potent anti-proliferative and anti-inflammatory effects in neoplastic illness conditions. LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466574/ https://www.ncbi.nlm.nih.gov/pubmed/34572719 http://dx.doi.org/10.3390/antibiotics10091136 |
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author | Abdel-Salam, Mostafa A. L. Pinto, Bárbara Cassali, Geovanni Bueno, Lilian Pêgas, Gabriela Oliveira, Fabrício Silva, Irismara Klein, André de Souza-Fagundes, Elaine Maria de Lima, Maria Elena Carvalho-Tavares, Juliana |
author_facet | Abdel-Salam, Mostafa A. L. Pinto, Bárbara Cassali, Geovanni Bueno, Lilian Pêgas, Gabriela Oliveira, Fabrício Silva, Irismara Klein, André de Souza-Fagundes, Elaine Maria de Lima, Maria Elena Carvalho-Tavares, Juliana |
author_sort | Abdel-Salam, Mostafa A. L. |
collection | PubMed |
description | Cationic anticancer peptides have exhibited potent anti-proliferative and anti-inflammatory effects in neoplastic illness conditions. LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on the effects of LyeTxI-b on a 4T1 mouse mammary carcinoma model. Mice with a palpable tumor in the left flank were subcutaneously or intratumorally injected with LyeTx I-b (5 mg/kg), which significantly decreased the tumor volume and metastatic nodules. Histological analyses showed a large necrotic area in treated primary tumors compared to the control. LyeTxI-b reduced tumor growth and lung metastasis in the 4T1 mouse mammary carcinoma model with no signs of toxicity in healthy or cancerous mice. The mechanism of action of LyeTx I-b on the 4T1 mouse mammary carcinoma model was evaluated in vitro and is associated with induction of apoptosis and cell proliferation inhibition. Furthermore, LyeTx I-b seems to be an efficient regulator of the 4T1 tumor microenvironment by modulating several cytokines, such as TGF-β, TNF-α, IL-1β, IL-6, and IL-10, in primary tumor and lung, spleen, and brain. LyeTx I-b also plays a role in leukocytes rolling and adhesion into spinal cord microcirculation and in the number of circulating leukocytes. These data suggest a potent antineoplastic efficacy ofLyeTx I-b. |
format | Online Article Text |
id | pubmed-8466574 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84665742021-09-27 LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model Abdel-Salam, Mostafa A. L. Pinto, Bárbara Cassali, Geovanni Bueno, Lilian Pêgas, Gabriela Oliveira, Fabrício Silva, Irismara Klein, André de Souza-Fagundes, Elaine Maria de Lima, Maria Elena Carvalho-Tavares, Juliana Antibiotics (Basel) Article Cationic anticancer peptides have exhibited potent anti-proliferative and anti-inflammatory effects in neoplastic illness conditions. LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on the effects of LyeTxI-b on a 4T1 mouse mammary carcinoma model. Mice with a palpable tumor in the left flank were subcutaneously or intratumorally injected with LyeTx I-b (5 mg/kg), which significantly decreased the tumor volume and metastatic nodules. Histological analyses showed a large necrotic area in treated primary tumors compared to the control. LyeTxI-b reduced tumor growth and lung metastasis in the 4T1 mouse mammary carcinoma model with no signs of toxicity in healthy or cancerous mice. The mechanism of action of LyeTx I-b on the 4T1 mouse mammary carcinoma model was evaluated in vitro and is associated with induction of apoptosis and cell proliferation inhibition. Furthermore, LyeTx I-b seems to be an efficient regulator of the 4T1 tumor microenvironment by modulating several cytokines, such as TGF-β, TNF-α, IL-1β, IL-6, and IL-10, in primary tumor and lung, spleen, and brain. LyeTx I-b also plays a role in leukocytes rolling and adhesion into spinal cord microcirculation and in the number of circulating leukocytes. These data suggest a potent antineoplastic efficacy ofLyeTx I-b. MDPI 2021-09-21 /pmc/articles/PMC8466574/ /pubmed/34572719 http://dx.doi.org/10.3390/antibiotics10091136 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Abdel-Salam, Mostafa A. L. Pinto, Bárbara Cassali, Geovanni Bueno, Lilian Pêgas, Gabriela Oliveira, Fabrício Silva, Irismara Klein, André de Souza-Fagundes, Elaine Maria de Lima, Maria Elena Carvalho-Tavares, Juliana LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model |
title | LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model |
title_full | LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model |
title_fullStr | LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model |
title_full_unstemmed | LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model |
title_short | LyeTx I-b Peptide Attenuates Tumor Burden and Metastasis in a Mouse 4T1 Breast Cancer Model |
title_sort | lyetx i-b peptide attenuates tumor burden and metastasis in a mouse 4t1 breast cancer model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466574/ https://www.ncbi.nlm.nih.gov/pubmed/34572719 http://dx.doi.org/10.3390/antibiotics10091136 |
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