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Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect

Phaeodactylum tricornutum is a rich source of fucoxanthin, a carotenoid with several health benefits. In the present study, high performance countercurrent chromatography (HPCCC) was used to isolate fucoxanthin from an extract of P. tricornutum. A multiple sequential injection HPCCC method was devel...

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Autores principales: Bárcenas-Pérez, Daniela, Střížek, Antonín, Hrouzek, Pavel, Kopecký, Jiří, Barradas, Marta, Sierra-Ramirez, Arantzazu, Fernandez-Marcos, Pablo J., Cheel, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466784/
https://www.ncbi.nlm.nih.gov/pubmed/34564179
http://dx.doi.org/10.3390/md19090517
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author Bárcenas-Pérez, Daniela
Střížek, Antonín
Hrouzek, Pavel
Kopecký, Jiří
Barradas, Marta
Sierra-Ramirez, Arantzazu
Fernandez-Marcos, Pablo J.
Cheel, José
author_facet Bárcenas-Pérez, Daniela
Střížek, Antonín
Hrouzek, Pavel
Kopecký, Jiří
Barradas, Marta
Sierra-Ramirez, Arantzazu
Fernandez-Marcos, Pablo J.
Cheel, José
author_sort Bárcenas-Pérez, Daniela
collection PubMed
description Phaeodactylum tricornutum is a rich source of fucoxanthin, a carotenoid with several health benefits. In the present study, high performance countercurrent chromatography (HPCCC) was used to isolate fucoxanthin from an extract of P. tricornutum. A multiple sequential injection HPCCC method was developed combining two elution modes (reverse phase and extrusion). The lower phase of a biphasic solvent system (n-heptane, ethyl acetate, ethanol and water, ratio 5/5/6/3, v/v/v/v) was used as the mobile phase, while the upper phase was the stationary phase. Ten consecutive sample injections (240 mg of extract each) were performed leading to the separation of 38 mg fucoxanthin with purity of 97% and a recovery of 98%. The process throughput was 0.189 g/h, while the efficiency per gram of fucoxanthin was 0.003 g/h. Environmental risk and general process evaluation factors were used for assessment of the developed separation method and compared with existing fucoxanthin liquid-liquid isolation methods. The isolated fucoxanthin retained its well-described ability to induce nuclear translocation of transcription factor FOXO3. Overall, the developed isolation method may represent a useful model to produce biologically active fucoxanthin from diatom biomass.
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spelling pubmed-84667842021-09-27 Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect Bárcenas-Pérez, Daniela Střížek, Antonín Hrouzek, Pavel Kopecký, Jiří Barradas, Marta Sierra-Ramirez, Arantzazu Fernandez-Marcos, Pablo J. Cheel, José Mar Drugs Article Phaeodactylum tricornutum is a rich source of fucoxanthin, a carotenoid with several health benefits. In the present study, high performance countercurrent chromatography (HPCCC) was used to isolate fucoxanthin from an extract of P. tricornutum. A multiple sequential injection HPCCC method was developed combining two elution modes (reverse phase and extrusion). The lower phase of a biphasic solvent system (n-heptane, ethyl acetate, ethanol and water, ratio 5/5/6/3, v/v/v/v) was used as the mobile phase, while the upper phase was the stationary phase. Ten consecutive sample injections (240 mg of extract each) were performed leading to the separation of 38 mg fucoxanthin with purity of 97% and a recovery of 98%. The process throughput was 0.189 g/h, while the efficiency per gram of fucoxanthin was 0.003 g/h. Environmental risk and general process evaluation factors were used for assessment of the developed separation method and compared with existing fucoxanthin liquid-liquid isolation methods. The isolated fucoxanthin retained its well-described ability to induce nuclear translocation of transcription factor FOXO3. Overall, the developed isolation method may represent a useful model to produce biologically active fucoxanthin from diatom biomass. MDPI 2021-09-11 /pmc/articles/PMC8466784/ /pubmed/34564179 http://dx.doi.org/10.3390/md19090517 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bárcenas-Pérez, Daniela
Střížek, Antonín
Hrouzek, Pavel
Kopecký, Jiří
Barradas, Marta
Sierra-Ramirez, Arantzazu
Fernandez-Marcos, Pablo J.
Cheel, José
Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
title Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
title_full Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
title_fullStr Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
title_full_unstemmed Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
title_short Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
title_sort production of fucoxanthin from phaeodactylum tricornutum using high performance countercurrent chromatography retaining its foxo3 nuclear translocation-inducing effect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466784/
https://www.ncbi.nlm.nih.gov/pubmed/34564179
http://dx.doi.org/10.3390/md19090517
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