Cargando…
The Insights into Mitochondrial Genomes of Sunflowers
The significant difference in the mtDNA size and structure with simultaneous slow evolving genes makes the mitochondrial genome paradoxical among all three DNA carriers in the plant cell. Such features make mitochondrial genome investigations of particular interest. The genus Helianthus is a diverse...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466785/ https://www.ncbi.nlm.nih.gov/pubmed/34579307 http://dx.doi.org/10.3390/plants10091774 |
_version_ | 1784573228760432640 |
---|---|
author | Makarenko, Maksim S. Omelchenko, Denis O. Usatov, Alexander V. Gavrilova, Vera A. |
author_facet | Makarenko, Maksim S. Omelchenko, Denis O. Usatov, Alexander V. Gavrilova, Vera A. |
author_sort | Makarenko, Maksim S. |
collection | PubMed |
description | The significant difference in the mtDNA size and structure with simultaneous slow evolving genes makes the mitochondrial genome paradoxical among all three DNA carriers in the plant cell. Such features make mitochondrial genome investigations of particular interest. The genus Helianthus is a diverse taxonomic group, including at least two economically valuable species—common sunflower (H. annuus) and Jerusalem artichoke (H. tuberosus). The successful investigation of the sunflower nuclear genome provided insights into some genomics aspects and significantly intensified sunflower genetic studies. However, the investigations of organelles’ genetic information in Helianthus, especially devoted to mitochondrial genomics, are presented by limited studies. Using NGS sequencing, we assembled the complete mitochondrial genomes for H. occidentalis (281,175 bp) and H. tuberosus (281,287 bp) in the current investigation. Besides the master circle chromosome, in the case of H. tuberosus, the 1361 bp circular plasmid was identified. The mitochondrial gene content was found to be identical for both sunflower species, counting 32 protein-coding genes, 3 rRNA, 23 tRNA genes, and 18 ORFs. The comparative analysis between perennial sunflowers revealed common and polymorphic SSR and SNPs. Comparison of perennial sunflowers with H. annuus allowed us to establish similar rearrangements in mitogenomes, which have possibly been inherited from a common ancestor after the divergence of annual and perennial sunflower species. It is notable that H. occidentalis and H. tuberosus mitogenomes are much more similar to H. strumosus than H. grosseserratus. |
format | Online Article Text |
id | pubmed-8466785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84667852021-09-27 The Insights into Mitochondrial Genomes of Sunflowers Makarenko, Maksim S. Omelchenko, Denis O. Usatov, Alexander V. Gavrilova, Vera A. Plants (Basel) Article The significant difference in the mtDNA size and structure with simultaneous slow evolving genes makes the mitochondrial genome paradoxical among all three DNA carriers in the plant cell. Such features make mitochondrial genome investigations of particular interest. The genus Helianthus is a diverse taxonomic group, including at least two economically valuable species—common sunflower (H. annuus) and Jerusalem artichoke (H. tuberosus). The successful investigation of the sunflower nuclear genome provided insights into some genomics aspects and significantly intensified sunflower genetic studies. However, the investigations of organelles’ genetic information in Helianthus, especially devoted to mitochondrial genomics, are presented by limited studies. Using NGS sequencing, we assembled the complete mitochondrial genomes for H. occidentalis (281,175 bp) and H. tuberosus (281,287 bp) in the current investigation. Besides the master circle chromosome, in the case of H. tuberosus, the 1361 bp circular plasmid was identified. The mitochondrial gene content was found to be identical for both sunflower species, counting 32 protein-coding genes, 3 rRNA, 23 tRNA genes, and 18 ORFs. The comparative analysis between perennial sunflowers revealed common and polymorphic SSR and SNPs. Comparison of perennial sunflowers with H. annuus allowed us to establish similar rearrangements in mitogenomes, which have possibly been inherited from a common ancestor after the divergence of annual and perennial sunflower species. It is notable that H. occidentalis and H. tuberosus mitogenomes are much more similar to H. strumosus than H. grosseserratus. MDPI 2021-08-26 /pmc/articles/PMC8466785/ /pubmed/34579307 http://dx.doi.org/10.3390/plants10091774 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Makarenko, Maksim S. Omelchenko, Denis O. Usatov, Alexander V. Gavrilova, Vera A. The Insights into Mitochondrial Genomes of Sunflowers |
title | The Insights into Mitochondrial Genomes of Sunflowers |
title_full | The Insights into Mitochondrial Genomes of Sunflowers |
title_fullStr | The Insights into Mitochondrial Genomes of Sunflowers |
title_full_unstemmed | The Insights into Mitochondrial Genomes of Sunflowers |
title_short | The Insights into Mitochondrial Genomes of Sunflowers |
title_sort | insights into mitochondrial genomes of sunflowers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466785/ https://www.ncbi.nlm.nih.gov/pubmed/34579307 http://dx.doi.org/10.3390/plants10091774 |
work_keys_str_mv | AT makarenkomaksims theinsightsintomitochondrialgenomesofsunflowers AT omelchenkodeniso theinsightsintomitochondrialgenomesofsunflowers AT usatovalexanderv theinsightsintomitochondrialgenomesofsunflowers AT gavrilovaveraa theinsightsintomitochondrialgenomesofsunflowers AT makarenkomaksims insightsintomitochondrialgenomesofsunflowers AT omelchenkodeniso insightsintomitochondrialgenomesofsunflowers AT usatovalexanderv insightsintomitochondrialgenomesofsunflowers AT gavrilovaveraa insightsintomitochondrialgenomesofsunflowers |