Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL

The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained b...

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Autores principales: Sigida, Elena N., Ibrahim, Ibrahim M., Kokoulin, Maxim S., Abulreesh, Hussein H., Elbanna, Khaled, Konnova, Svetlana A., Fedonenko, Yulia P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466920/
https://www.ncbi.nlm.nih.gov/pubmed/34564170
http://dx.doi.org/10.3390/md19090508
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author Sigida, Elena N.
Ibrahim, Ibrahim M.
Kokoulin, Maxim S.
Abulreesh, Hussein H.
Elbanna, Khaled
Konnova, Svetlana A.
Fedonenko, Yulia P.
author_facet Sigida, Elena N.
Ibrahim, Ibrahim M.
Kokoulin, Maxim S.
Abulreesh, Hussein H.
Elbanna, Khaled
Konnova, Svetlana A.
Fedonenko, Yulia P.
author_sort Sigida, Elena N.
collection PubMed
description The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, including (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Manp4Lac-(1→3)-α-ManpNAc-(1→3)-β-Rhap-(1→4)-α-GlcpNAc-(1→, where Manp4Lac is 4-O-[1-carboxyethyl]mannose.
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spelling pubmed-84669202021-09-27 Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL Sigida, Elena N. Ibrahim, Ibrahim M. Kokoulin, Maxim S. Abulreesh, Hussein H. Elbanna, Khaled Konnova, Svetlana A. Fedonenko, Yulia P. Mar Drugs Article The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, including (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Manp4Lac-(1→3)-α-ManpNAc-(1→3)-β-Rhap-(1→4)-α-GlcpNAc-(1→, where Manp4Lac is 4-O-[1-carboxyethyl]mannose. MDPI 2021-09-07 /pmc/articles/PMC8466920/ /pubmed/34564170 http://dx.doi.org/10.3390/md19090508 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sigida, Elena N.
Ibrahim, Ibrahim M.
Kokoulin, Maxim S.
Abulreesh, Hussein H.
Elbanna, Khaled
Konnova, Svetlana A.
Fedonenko, Yulia P.
Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL
title Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL
title_full Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL
title_fullStr Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL
title_full_unstemmed Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL
title_short Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL
title_sort structure of the 4-o-[1-carboxyethyl]-d-mannose-containing o-specific polysaccharide of a halophilic bacterium salinivibrio sp. eg9s8ql
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466920/
https://www.ncbi.nlm.nih.gov/pubmed/34564170
http://dx.doi.org/10.3390/md19090508
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