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Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka

The aim of the study was to identify canine parvovirus type 2 (CPV-2) subtypes circulating among a selected population of domestic dogs and cats in Sri Lanka and to investigate the evolutionary patterns among Sri Lankan viruses in the context of contemporary global CPV-2 sequences. Altogether, 40/61...

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Autores principales: Jinadasa, Rasika, Ghosh, Sayani, Hills, Simon, Premalal, Thushini, Atapattu, Ushani, Fuward, Manohari, Kalupahana, Wasantha, Dunowska, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466987/
https://www.ncbi.nlm.nih.gov/pubmed/34578133
http://dx.doi.org/10.3390/pathogens10091102
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author Jinadasa, Rasika
Ghosh, Sayani
Hills, Simon
Premalal, Thushini
Atapattu, Ushani
Fuward, Manohari
Kalupahana, Wasantha
Dunowska, Magdalena
author_facet Jinadasa, Rasika
Ghosh, Sayani
Hills, Simon
Premalal, Thushini
Atapattu, Ushani
Fuward, Manohari
Kalupahana, Wasantha
Dunowska, Magdalena
author_sort Jinadasa, Rasika
collection PubMed
description The aim of the study was to identify canine parvovirus type 2 (CPV-2) subtypes circulating among a selected population of domestic dogs and cats in Sri Lanka and to investigate the evolutionary patterns among Sri Lankan viruses in the context of contemporary global CPV-2 sequences. Altogether, 40/61 (65.6%) samples tested were positive for CPV-2 DNA, including 31/48 (64.6%) dogs and 9/13 (69%) cats. All three subtypes (CPV-2a, CPV-2b and CPV-2c) were detected, with CPV-2a being most common. International median joining haplotype network of 291 CPV-2 sequences suggested that there was little barrier for CPV-2 moving between different geographical regions worldwide, including Sri Lanka, and that there was no correlation between the genetic structure within the molecular network and the decade of sample collection. By contrast, there was correlation between CPV-2 subtype and genetic structure, both within the international network and within the network built from 31 Sri Lankan CPV-2 sequences only. The structure within the latter was not correlated with the location of the veterinary clinic where the samples were submitted, the age or species of the host. Altogether, we have shown that there is considerable variability of CPV-2 genotypes circulating in Sri Lanka, which is likely driven by both local evolution and introduction from other countries. The similarity of CPV-2 obtained from cats and dogs suggests that cats may play a role in the epidemiology of CPV-2 in Sri Lanka.
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spelling pubmed-84669872021-09-27 Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka Jinadasa, Rasika Ghosh, Sayani Hills, Simon Premalal, Thushini Atapattu, Ushani Fuward, Manohari Kalupahana, Wasantha Dunowska, Magdalena Pathogens Article The aim of the study was to identify canine parvovirus type 2 (CPV-2) subtypes circulating among a selected population of domestic dogs and cats in Sri Lanka and to investigate the evolutionary patterns among Sri Lankan viruses in the context of contemporary global CPV-2 sequences. Altogether, 40/61 (65.6%) samples tested were positive for CPV-2 DNA, including 31/48 (64.6%) dogs and 9/13 (69%) cats. All three subtypes (CPV-2a, CPV-2b and CPV-2c) were detected, with CPV-2a being most common. International median joining haplotype network of 291 CPV-2 sequences suggested that there was little barrier for CPV-2 moving between different geographical regions worldwide, including Sri Lanka, and that there was no correlation between the genetic structure within the molecular network and the decade of sample collection. By contrast, there was correlation between CPV-2 subtype and genetic structure, both within the international network and within the network built from 31 Sri Lankan CPV-2 sequences only. The structure within the latter was not correlated with the location of the veterinary clinic where the samples were submitted, the age or species of the host. Altogether, we have shown that there is considerable variability of CPV-2 genotypes circulating in Sri Lanka, which is likely driven by both local evolution and introduction from other countries. The similarity of CPV-2 obtained from cats and dogs suggests that cats may play a role in the epidemiology of CPV-2 in Sri Lanka. MDPI 2021-08-29 /pmc/articles/PMC8466987/ /pubmed/34578133 http://dx.doi.org/10.3390/pathogens10091102 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jinadasa, Rasika
Ghosh, Sayani
Hills, Simon
Premalal, Thushini
Atapattu, Ushani
Fuward, Manohari
Kalupahana, Wasantha
Dunowska, Magdalena
Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka
title Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka
title_full Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka
title_fullStr Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka
title_full_unstemmed Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka
title_short Genomic Variability of Canine Parvoviruses from a Selected Population of Dogs and Cats in Sri Lanka
title_sort genomic variability of canine parvoviruses from a selected population of dogs and cats in sri lanka
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466987/
https://www.ncbi.nlm.nih.gov/pubmed/34578133
http://dx.doi.org/10.3390/pathogens10091102
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