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A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level

Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictivene...

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Autores principales: Schneider, Verena, Kruse, Daniel, de Mattos, Ives Bernardelli, Zöphel, Saskia, Tiltmann, Kendra-Kathrin, Reigl, Amelie, Khan, Sarah, Funk, Martin, Bodenschatz, Karl, Groeber-Becker, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466997/
https://www.ncbi.nlm.nih.gov/pubmed/34572338
http://dx.doi.org/10.3390/biomedicines9091153
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author Schneider, Verena
Kruse, Daniel
de Mattos, Ives Bernardelli
Zöphel, Saskia
Tiltmann, Kendra-Kathrin
Reigl, Amelie
Khan, Sarah
Funk, Martin
Bodenschatz, Karl
Groeber-Becker, Florian
author_facet Schneider, Verena
Kruse, Daniel
de Mattos, Ives Bernardelli
Zöphel, Saskia
Tiltmann, Kendra-Kathrin
Reigl, Amelie
Khan, Sarah
Funk, Martin
Bodenschatz, Karl
Groeber-Becker, Florian
author_sort Schneider, Verena
collection PubMed
description Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.
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spelling pubmed-84669972021-09-27 A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level Schneider, Verena Kruse, Daniel de Mattos, Ives Bernardelli Zöphel, Saskia Tiltmann, Kendra-Kathrin Reigl, Amelie Khan, Sarah Funk, Martin Bodenschatz, Karl Groeber-Becker, Florian Biomedicines Article Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level. MDPI 2021-09-03 /pmc/articles/PMC8466997/ /pubmed/34572338 http://dx.doi.org/10.3390/biomedicines9091153 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schneider, Verena
Kruse, Daniel
de Mattos, Ives Bernardelli
Zöphel, Saskia
Tiltmann, Kendra-Kathrin
Reigl, Amelie
Khan, Sarah
Funk, Martin
Bodenschatz, Karl
Groeber-Becker, Florian
A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level
title A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level
title_full A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level
title_fullStr A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level
title_full_unstemmed A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level
title_short A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level
title_sort 3d in vitro model for burn wounds: monitoring of regeneration on the epidermal level
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466997/
https://www.ncbi.nlm.nih.gov/pubmed/34572338
http://dx.doi.org/10.3390/biomedicines9091153
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