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Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype
Dengue virus type 2 (DENV-2) is, traditionally, the most studied serotype due to its association with explosive outbreaks and severe cases. In Brazil, almost 20 years after the first introduction in the 1990s, a new lineage (Lineage II) of the DENV-2 Asian/American genotype emerged and caused an epi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467194/ https://www.ncbi.nlm.nih.gov/pubmed/34578117 http://dx.doi.org/10.3390/pathogens10091084 |
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author | Jácome, Fernanda Cunha Caldas, Gabriela Cardoso Rasinhas, Arthur da Costa de Almeida, Ana Luisa Teixeira de Souza, Daniel Dias Coutinho Paulino, Amanda Carlos da Silva, Marcos Alexandre Nunes Barth, Ortrud Monika dos Santos, Flávia Barreto Barreto-Vieira, Debora Ferreira |
author_facet | Jácome, Fernanda Cunha Caldas, Gabriela Cardoso Rasinhas, Arthur da Costa de Almeida, Ana Luisa Teixeira de Souza, Daniel Dias Coutinho Paulino, Amanda Carlos da Silva, Marcos Alexandre Nunes Barth, Ortrud Monika dos Santos, Flávia Barreto Barreto-Vieira, Debora Ferreira |
author_sort | Jácome, Fernanda Cunha |
collection | PubMed |
description | Dengue virus type 2 (DENV-2) is, traditionally, the most studied serotype due to its association with explosive outbreaks and severe cases. In Brazil, almost 20 years after the first introduction in the 1990s, a new lineage (Lineage II) of the DENV-2 Asian/American genotype emerged and caused an epidemic with severe cases and hospitalizations. Severe dengue includes multiple organ failure, and renal involvement can be potentially related to increased mortality. In order to better understand the role of DENV infection in renal injury, here we aimed to investigate the outcomes of infection with two distinct lineages of DENV-2 Asian/American genotype in the kidney of a murine model. BALB/c mice were infected with Lineages I and II and tissues were submitted to histopathology, immunohistochemistry, histomorphometry and ultrastructural analysis. Blood urea nitrogen (BUN) was detected in blood sample accessed by cardiac puncture. A tendency in kidney weight increase was observed in mice infected with both lineages, but urea levels, on average, were increased only in mice infected with Lineage II. The DENV antigen was detected in the tissue of mice infected with Lineage II and morphological changes were similar to those observed in human dengue cases. Furthermore, the parameters such as organ weight, urea levels and morphometric analysis, showed significant differences between the two lineages in the infected BALB/c, which was demonstrated to be a suitable experimental model for dengue pathophysiology studies in kidneys. |
format | Online Article Text |
id | pubmed-8467194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84671942021-09-27 Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype Jácome, Fernanda Cunha Caldas, Gabriela Cardoso Rasinhas, Arthur da Costa de Almeida, Ana Luisa Teixeira de Souza, Daniel Dias Coutinho Paulino, Amanda Carlos da Silva, Marcos Alexandre Nunes Barth, Ortrud Monika dos Santos, Flávia Barreto Barreto-Vieira, Debora Ferreira Pathogens Article Dengue virus type 2 (DENV-2) is, traditionally, the most studied serotype due to its association with explosive outbreaks and severe cases. In Brazil, almost 20 years after the first introduction in the 1990s, a new lineage (Lineage II) of the DENV-2 Asian/American genotype emerged and caused an epidemic with severe cases and hospitalizations. Severe dengue includes multiple organ failure, and renal involvement can be potentially related to increased mortality. In order to better understand the role of DENV infection in renal injury, here we aimed to investigate the outcomes of infection with two distinct lineages of DENV-2 Asian/American genotype in the kidney of a murine model. BALB/c mice were infected with Lineages I and II and tissues were submitted to histopathology, immunohistochemistry, histomorphometry and ultrastructural analysis. Blood urea nitrogen (BUN) was detected in blood sample accessed by cardiac puncture. A tendency in kidney weight increase was observed in mice infected with both lineages, but urea levels, on average, were increased only in mice infected with Lineage II. The DENV antigen was detected in the tissue of mice infected with Lineage II and morphological changes were similar to those observed in human dengue cases. Furthermore, the parameters such as organ weight, urea levels and morphometric analysis, showed significant differences between the two lineages in the infected BALB/c, which was demonstrated to be a suitable experimental model for dengue pathophysiology studies in kidneys. MDPI 2021-08-26 /pmc/articles/PMC8467194/ /pubmed/34578117 http://dx.doi.org/10.3390/pathogens10091084 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jácome, Fernanda Cunha Caldas, Gabriela Cardoso Rasinhas, Arthur da Costa de Almeida, Ana Luisa Teixeira de Souza, Daniel Dias Coutinho Paulino, Amanda Carlos da Silva, Marcos Alexandre Nunes Barth, Ortrud Monika dos Santos, Flávia Barreto Barreto-Vieira, Debora Ferreira Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype |
title | Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype |
title_full | Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype |
title_fullStr | Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype |
title_full_unstemmed | Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype |
title_short | Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype |
title_sort | brazilian dengue virus type 2-associated renal involvement in a murine model: outcomes after infection by two lineages of the asian/american genotype |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467194/ https://www.ncbi.nlm.nih.gov/pubmed/34578117 http://dx.doi.org/10.3390/pathogens10091084 |
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