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High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans

Radiation damages many cellular components and disrupts cellular functions, and was previously reported to impair locomotion in the model organism Caenorhabditis elegans. However, the response to even higher doses is not clear. First, to investigate the effects of high-dose radiation on the locomoti...

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Autores principales: Yamasaki, Akira, Suzuki, Michiyo, Funayama, Tomoo, Moriwaki, Takahito, Sakashita, Tetsuya, Kobayashi, Yasuhiko, Zhang-Akiyama, Qiu-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467272/
https://www.ncbi.nlm.nih.gov/pubmed/34575973
http://dx.doi.org/10.3390/ijms22189810
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author Yamasaki, Akira
Suzuki, Michiyo
Funayama, Tomoo
Moriwaki, Takahito
Sakashita, Tetsuya
Kobayashi, Yasuhiko
Zhang-Akiyama, Qiu-Mei
author_facet Yamasaki, Akira
Suzuki, Michiyo
Funayama, Tomoo
Moriwaki, Takahito
Sakashita, Tetsuya
Kobayashi, Yasuhiko
Zhang-Akiyama, Qiu-Mei
author_sort Yamasaki, Akira
collection PubMed
description Radiation damages many cellular components and disrupts cellular functions, and was previously reported to impair locomotion in the model organism Caenorhabditis elegans. However, the response to even higher doses is not clear. First, to investigate the effects of high-dose radiation on the locomotion of C. elegans, we investigated the dose range that reduces whole-body locomotion or leads to death. Irradiation was performed in the range of 0–6 kGy. In the crawling analysis, motility decreased after irradiation in a dose-dependent manner. Exposure to 6 kGy of radiation affected crawling on agar immediately and caused the complete loss of motility. Both γ-rays and carbon-ion beams significantly reduced crawling motility at 3 kGy. Next, swimming in buffer was measured as a motility index to assess the response over time after irradiation and motility similarly decreased. However, swimming partially recovered 6 h after irradiation with 3 kGy of γ-rays. To examine the possibility of a recovery mechanism, in situ GFP reporter assay of the autophagy-related gene lgg-1 was performed. The fluorescence intensity was stronger in the anterior half of the body 7 h after irradiation with 3 kGy of γ-rays. GFP::LGG-1 induction was observed in the pharynx, neurons along the body, and the intestine. Furthermore, worms were exposed to region-specific radiation with carbon-ion microbeams and the trajectory of crawling was measured by image processing. Motility was lower after anterior-half body irradiation than after posterior-half body irradiation. This further supported that the anterior half of the body is important in the locomotory response to radiation.
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spelling pubmed-84672722021-09-27 High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans Yamasaki, Akira Suzuki, Michiyo Funayama, Tomoo Moriwaki, Takahito Sakashita, Tetsuya Kobayashi, Yasuhiko Zhang-Akiyama, Qiu-Mei Int J Mol Sci Article Radiation damages many cellular components and disrupts cellular functions, and was previously reported to impair locomotion in the model organism Caenorhabditis elegans. However, the response to even higher doses is not clear. First, to investigate the effects of high-dose radiation on the locomotion of C. elegans, we investigated the dose range that reduces whole-body locomotion or leads to death. Irradiation was performed in the range of 0–6 kGy. In the crawling analysis, motility decreased after irradiation in a dose-dependent manner. Exposure to 6 kGy of radiation affected crawling on agar immediately and caused the complete loss of motility. Both γ-rays and carbon-ion beams significantly reduced crawling motility at 3 kGy. Next, swimming in buffer was measured as a motility index to assess the response over time after irradiation and motility similarly decreased. However, swimming partially recovered 6 h after irradiation with 3 kGy of γ-rays. To examine the possibility of a recovery mechanism, in situ GFP reporter assay of the autophagy-related gene lgg-1 was performed. The fluorescence intensity was stronger in the anterior half of the body 7 h after irradiation with 3 kGy of γ-rays. GFP::LGG-1 induction was observed in the pharynx, neurons along the body, and the intestine. Furthermore, worms were exposed to region-specific radiation with carbon-ion microbeams and the trajectory of crawling was measured by image processing. Motility was lower after anterior-half body irradiation than after posterior-half body irradiation. This further supported that the anterior half of the body is important in the locomotory response to radiation. MDPI 2021-09-10 /pmc/articles/PMC8467272/ /pubmed/34575973 http://dx.doi.org/10.3390/ijms22189810 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamasaki, Akira
Suzuki, Michiyo
Funayama, Tomoo
Moriwaki, Takahito
Sakashita, Tetsuya
Kobayashi, Yasuhiko
Zhang-Akiyama, Qiu-Mei
High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans
title High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans
title_full High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans
title_fullStr High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans
title_full_unstemmed High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans
title_short High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans
title_sort high-dose irradiation inhibits motility and induces autophagy in caenorhabditis elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467272/
https://www.ncbi.nlm.nih.gov/pubmed/34575973
http://dx.doi.org/10.3390/ijms22189810
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