Cargando…

Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils

Recently, the herbal compress was successfully developed and applied for cellulite treatment. The aim of this study was to formulate a more convenient dosage form of herbal application from the original formula. In addition, we aimed to characterize and evaluate the stability of the developed dosage...

Descripción completa

Detalles Bibliográficos
Autores principales: Ngamdokmai, Ngamrayu, Ingkaninan, Kornkanok, Chaichamnong, Nattiya, Chootip, Krongkarn, Neungchamnong, Nitra, Waranuch, Neti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467277/
https://www.ncbi.nlm.nih.gov/pubmed/34577542
http://dx.doi.org/10.3390/ph14090842
_version_ 1784573356186533888
author Ngamdokmai, Ngamrayu
Ingkaninan, Kornkanok
Chaichamnong, Nattiya
Chootip, Krongkarn
Neungchamnong, Nitra
Waranuch, Neti
author_facet Ngamdokmai, Ngamrayu
Ingkaninan, Kornkanok
Chaichamnong, Nattiya
Chootip, Krongkarn
Neungchamnong, Nitra
Waranuch, Neti
author_sort Ngamdokmai, Ngamrayu
collection PubMed
description Recently, the herbal compress was successfully developed and applied for cellulite treatment. The aim of this study was to formulate a more convenient dosage form of herbal application from the original formula. In addition, we aimed to characterize and evaluate the stability of the developed dosage form. A gelled emulsion, or an “emgel,” incorporated with 0.1 wt% tea and coffee extracts (1:1 ratio) plus 5 wt% essential oils (mixed oil) was prepared. The caffeine content in the finished product obtained from tea and coffee extracts analyzed by HPLC was 48.1 ± 2.3 µg/g. The bio-active marker monoterpenes of mixed oil characterized by headspace GCMS were camphene 50.8 ± 1.8 µg/mg, camphor 251.0 ± 3.2 µg/mg, 3-carene 46.7 ± 1.8 µg/mg, α-citral 75.0 ± 2.1 µg/mg, β-citral 65.6 ± 1.3 µg/mg, limonene 36.8 ± 6.7 µg/mg, myrcene 53.3 ± 4.5 µg/mg, α-pinene 85.2 ± 0.6 µg/mg, β-pinene 88.4 ± 1.1 µg/mg, and terpinene-4-ol 104.3 ± 2.6 µg/mg. The stability study was carried out over a period of 3 months at 4, 25, and 50 °C. The caffeine content showed no significant changes and passed the acceptance criteria of ≥80% at all tested temperatures. However, monoterpenes showed their stability for only 2 months at 50 °C. Therefore, the shelf-life of the emgel was, consequently, calculated to be 31 months using the Q10 method. Thus, the anti-cellulite emgel was successfully formulated. The characterization methods and stability evaluation for caffeine and monoterpenes in an emgel matrix were also successfully developed and validated.
format Online
Article
Text
id pubmed-8467277
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84672772021-09-27 Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils Ngamdokmai, Ngamrayu Ingkaninan, Kornkanok Chaichamnong, Nattiya Chootip, Krongkarn Neungchamnong, Nitra Waranuch, Neti Pharmaceuticals (Basel) Article Recently, the herbal compress was successfully developed and applied for cellulite treatment. The aim of this study was to formulate a more convenient dosage form of herbal application from the original formula. In addition, we aimed to characterize and evaluate the stability of the developed dosage form. A gelled emulsion, or an “emgel,” incorporated with 0.1 wt% tea and coffee extracts (1:1 ratio) plus 5 wt% essential oils (mixed oil) was prepared. The caffeine content in the finished product obtained from tea and coffee extracts analyzed by HPLC was 48.1 ± 2.3 µg/g. The bio-active marker monoterpenes of mixed oil characterized by headspace GCMS were camphene 50.8 ± 1.8 µg/mg, camphor 251.0 ± 3.2 µg/mg, 3-carene 46.7 ± 1.8 µg/mg, α-citral 75.0 ± 2.1 µg/mg, β-citral 65.6 ± 1.3 µg/mg, limonene 36.8 ± 6.7 µg/mg, myrcene 53.3 ± 4.5 µg/mg, α-pinene 85.2 ± 0.6 µg/mg, β-pinene 88.4 ± 1.1 µg/mg, and terpinene-4-ol 104.3 ± 2.6 µg/mg. The stability study was carried out over a period of 3 months at 4, 25, and 50 °C. The caffeine content showed no significant changes and passed the acceptance criteria of ≥80% at all tested temperatures. However, monoterpenes showed their stability for only 2 months at 50 °C. Therefore, the shelf-life of the emgel was, consequently, calculated to be 31 months using the Q10 method. Thus, the anti-cellulite emgel was successfully formulated. The characterization methods and stability evaluation for caffeine and monoterpenes in an emgel matrix were also successfully developed and validated. MDPI 2021-08-25 /pmc/articles/PMC8467277/ /pubmed/34577542 http://dx.doi.org/10.3390/ph14090842 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ngamdokmai, Ngamrayu
Ingkaninan, Kornkanok
Chaichamnong, Nattiya
Chootip, Krongkarn
Neungchamnong, Nitra
Waranuch, Neti
Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils
title Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils
title_full Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils
title_fullStr Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils
title_full_unstemmed Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils
title_short Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils
title_sort development, characterization, and stability evaluation of the anti-cellulite emgel containing herbal extracts and essential oils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467277/
https://www.ncbi.nlm.nih.gov/pubmed/34577542
http://dx.doi.org/10.3390/ph14090842
work_keys_str_mv AT ngamdokmaingamrayu developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils
AT ingkaninankornkanok developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils
AT chaichamnongnattiya developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils
AT chootipkrongkarn developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils
AT neungchamnongnitra developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils
AT waranuchneti developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils