Cargando…
Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils
Recently, the herbal compress was successfully developed and applied for cellulite treatment. The aim of this study was to formulate a more convenient dosage form of herbal application from the original formula. In addition, we aimed to characterize and evaluate the stability of the developed dosage...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467277/ https://www.ncbi.nlm.nih.gov/pubmed/34577542 http://dx.doi.org/10.3390/ph14090842 |
_version_ | 1784573356186533888 |
---|---|
author | Ngamdokmai, Ngamrayu Ingkaninan, Kornkanok Chaichamnong, Nattiya Chootip, Krongkarn Neungchamnong, Nitra Waranuch, Neti |
author_facet | Ngamdokmai, Ngamrayu Ingkaninan, Kornkanok Chaichamnong, Nattiya Chootip, Krongkarn Neungchamnong, Nitra Waranuch, Neti |
author_sort | Ngamdokmai, Ngamrayu |
collection | PubMed |
description | Recently, the herbal compress was successfully developed and applied for cellulite treatment. The aim of this study was to formulate a more convenient dosage form of herbal application from the original formula. In addition, we aimed to characterize and evaluate the stability of the developed dosage form. A gelled emulsion, or an “emgel,” incorporated with 0.1 wt% tea and coffee extracts (1:1 ratio) plus 5 wt% essential oils (mixed oil) was prepared. The caffeine content in the finished product obtained from tea and coffee extracts analyzed by HPLC was 48.1 ± 2.3 µg/g. The bio-active marker monoterpenes of mixed oil characterized by headspace GCMS were camphene 50.8 ± 1.8 µg/mg, camphor 251.0 ± 3.2 µg/mg, 3-carene 46.7 ± 1.8 µg/mg, α-citral 75.0 ± 2.1 µg/mg, β-citral 65.6 ± 1.3 µg/mg, limonene 36.8 ± 6.7 µg/mg, myrcene 53.3 ± 4.5 µg/mg, α-pinene 85.2 ± 0.6 µg/mg, β-pinene 88.4 ± 1.1 µg/mg, and terpinene-4-ol 104.3 ± 2.6 µg/mg. The stability study was carried out over a period of 3 months at 4, 25, and 50 °C. The caffeine content showed no significant changes and passed the acceptance criteria of ≥80% at all tested temperatures. However, monoterpenes showed their stability for only 2 months at 50 °C. Therefore, the shelf-life of the emgel was, consequently, calculated to be 31 months using the Q10 method. Thus, the anti-cellulite emgel was successfully formulated. The characterization methods and stability evaluation for caffeine and monoterpenes in an emgel matrix were also successfully developed and validated. |
format | Online Article Text |
id | pubmed-8467277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84672772021-09-27 Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils Ngamdokmai, Ngamrayu Ingkaninan, Kornkanok Chaichamnong, Nattiya Chootip, Krongkarn Neungchamnong, Nitra Waranuch, Neti Pharmaceuticals (Basel) Article Recently, the herbal compress was successfully developed and applied for cellulite treatment. The aim of this study was to formulate a more convenient dosage form of herbal application from the original formula. In addition, we aimed to characterize and evaluate the stability of the developed dosage form. A gelled emulsion, or an “emgel,” incorporated with 0.1 wt% tea and coffee extracts (1:1 ratio) plus 5 wt% essential oils (mixed oil) was prepared. The caffeine content in the finished product obtained from tea and coffee extracts analyzed by HPLC was 48.1 ± 2.3 µg/g. The bio-active marker monoterpenes of mixed oil characterized by headspace GCMS were camphene 50.8 ± 1.8 µg/mg, camphor 251.0 ± 3.2 µg/mg, 3-carene 46.7 ± 1.8 µg/mg, α-citral 75.0 ± 2.1 µg/mg, β-citral 65.6 ± 1.3 µg/mg, limonene 36.8 ± 6.7 µg/mg, myrcene 53.3 ± 4.5 µg/mg, α-pinene 85.2 ± 0.6 µg/mg, β-pinene 88.4 ± 1.1 µg/mg, and terpinene-4-ol 104.3 ± 2.6 µg/mg. The stability study was carried out over a period of 3 months at 4, 25, and 50 °C. The caffeine content showed no significant changes and passed the acceptance criteria of ≥80% at all tested temperatures. However, monoterpenes showed their stability for only 2 months at 50 °C. Therefore, the shelf-life of the emgel was, consequently, calculated to be 31 months using the Q10 method. Thus, the anti-cellulite emgel was successfully formulated. The characterization methods and stability evaluation for caffeine and monoterpenes in an emgel matrix were also successfully developed and validated. MDPI 2021-08-25 /pmc/articles/PMC8467277/ /pubmed/34577542 http://dx.doi.org/10.3390/ph14090842 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ngamdokmai, Ngamrayu Ingkaninan, Kornkanok Chaichamnong, Nattiya Chootip, Krongkarn Neungchamnong, Nitra Waranuch, Neti Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils |
title | Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils |
title_full | Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils |
title_fullStr | Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils |
title_full_unstemmed | Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils |
title_short | Development, Characterization, and Stability Evaluation of the Anti-Cellulite Emgel Containing Herbal Extracts and Essential Oils |
title_sort | development, characterization, and stability evaluation of the anti-cellulite emgel containing herbal extracts and essential oils |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467277/ https://www.ncbi.nlm.nih.gov/pubmed/34577542 http://dx.doi.org/10.3390/ph14090842 |
work_keys_str_mv | AT ngamdokmaingamrayu developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils AT ingkaninankornkanok developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils AT chaichamnongnattiya developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils AT chootipkrongkarn developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils AT neungchamnongnitra developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils AT waranuchneti developmentcharacterizationandstabilityevaluationoftheanticelluliteemgelcontainingherbalextractsandessentialoils |