Cargando…

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm [Formula: see text] and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA buffer), a microheater for inc...

Descripción completa

Detalles Bibliográficos
Autores principales: Urbano-Gámez, Jesús David, Perdigones, Francisco, Quero, José Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467303/
https://www.ncbi.nlm.nih.gov/pubmed/34577715
http://dx.doi.org/10.3390/mi12091071
_version_ 1784573362957189120
author Urbano-Gámez, Jesús David
Perdigones, Francisco
Quero, José Manuel
author_facet Urbano-Gámez, Jesús David
Perdigones, Francisco
Quero, José Manuel
author_sort Urbano-Gámez, Jesús David
collection PubMed
description In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm [Formula: see text] and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA buffer), a microheater for increasing the solubility of the agarose, a negative temperature coefficient (NTC) thermistor for controlling the temperature, a light dependent resistor (LDR) sensor for measuring the transparency of the mixture, and two electrodes for performing the electrophoresis. The agarose preparation functions are governed by a microcontroller. The device requires a PMMA structure to define the wells of the agarose gel, and to release the electrodes from the agarose. The maximum voltage and current that the system requires are 40 V to perform the electrophoresis, and 1 A for activating the microheater. The chosen temperature for mixing is 80 [Formula: see text] C, with a mixing time of 10 min. In addition, the curing time is about 30 min. This device is intended to be integrated as a part of a larger lab-on-PCB system for DNA amplification and detection. However, it can be used to migrate DNA amplified in conventional thermocyclers. Moreover, the device can be modified for preparing larger agarose gels and performing electrophoresis.
format Online
Article
Text
id pubmed-8467303
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84673032021-09-27 Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications Urbano-Gámez, Jesús David Perdigones, Francisco Quero, José Manuel Micromachines (Basel) Article In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm [Formula: see text] and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA buffer), a microheater for increasing the solubility of the agarose, a negative temperature coefficient (NTC) thermistor for controlling the temperature, a light dependent resistor (LDR) sensor for measuring the transparency of the mixture, and two electrodes for performing the electrophoresis. The agarose preparation functions are governed by a microcontroller. The device requires a PMMA structure to define the wells of the agarose gel, and to release the electrodes from the agarose. The maximum voltage and current that the system requires are 40 V to perform the electrophoresis, and 1 A for activating the microheater. The chosen temperature for mixing is 80 [Formula: see text] C, with a mixing time of 10 min. In addition, the curing time is about 30 min. This device is intended to be integrated as a part of a larger lab-on-PCB system for DNA amplification and detection. However, it can be used to migrate DNA amplified in conventional thermocyclers. Moreover, the device can be modified for preparing larger agarose gels and performing electrophoresis. MDPI 2021-09-02 /pmc/articles/PMC8467303/ /pubmed/34577715 http://dx.doi.org/10.3390/mi12091071 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Urbano-Gámez, Jesús David
Perdigones, Francisco
Quero, José Manuel
Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications
title Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications
title_full Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications
title_fullStr Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications
title_full_unstemmed Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications
title_short Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications
title_sort semi-automatic lab-on-pcb system for agarose gel preparation and electrophoresis for biomedical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467303/
https://www.ncbi.nlm.nih.gov/pubmed/34577715
http://dx.doi.org/10.3390/mi12091071
work_keys_str_mv AT urbanogamezjesusdavid semiautomaticlabonpcbsystemforagarosegelpreparationandelectrophoresisforbiomedicalapplications
AT perdigonesfrancisco semiautomaticlabonpcbsystemforagarosegelpreparationandelectrophoresisforbiomedicalapplications
AT querojosemanuel semiautomaticlabonpcbsystemforagarosegelpreparationandelectrophoresisforbiomedicalapplications