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First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe

In the last decade, the cultivated area of maize has increased in Central Europe due to its high yield potential and diverse uses for feed and bio-energy. This has led to more intense maize cultivation, with narrowed crop rotations resulting in the increase in maize leaf diseases. During 2012 and 20...

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Autores principales: Ramos Romero, Lucia, Tacke, Dagmar, Koopmann, Birger, von Tiedemann, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467443/
https://www.ncbi.nlm.nih.gov/pubmed/34578248
http://dx.doi.org/10.3390/pathogens10091216
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author Ramos Romero, Lucia
Tacke, Dagmar
Koopmann, Birger
von Tiedemann, Andreas
author_facet Ramos Romero, Lucia
Tacke, Dagmar
Koopmann, Birger
von Tiedemann, Andreas
author_sort Ramos Romero, Lucia
collection PubMed
description In the last decade, the cultivated area of maize has increased in Central Europe due to its high yield potential and diverse uses for feed and bio-energy. This has led to more intense maize cultivation, with narrowed crop rotations resulting in the increase in maize leaf diseases. During 2012 and 2013, an inventory of maize leaf spot diseases was carried out in various regions in Central Europe. In addition to the major leaf pathogens, isolates of Phoma-like species were obtained from oval to elliptical spots on leaves or found in lesions produced by other leaf pathogens. A total of 16 representative Phoma-like strains were characterised for their pathogenicity on maize leaves, for their morphological characteristics and with a phylogenetic analysis based on multilocus sequence analysis using part of the actin (ACT), calmodulin (CAL), β-tubulin (TUB), internal transcribed spacer (ITS) region of ribosomal DNA and large subunit ribosomal RNA (LSU) genes. The strains were grouped into four clades, and morphological studies supported this classification for most of them. Strains were compared with six reference Phoma-like species strains from the Westerndijk Fungal Biodiversity Institute collection reported to colonise maize. The pathogenic group of strains from our collection (after completion of Koch’s postulates) did not cluster with any of these species, indicating a different and novel Phoma-like species infecting maize leaves. To our knowledge, this is the first study dissecting the Phoma species complex on maize leaves in Central Europe.
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spelling pubmed-84674432021-09-27 First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe Ramos Romero, Lucia Tacke, Dagmar Koopmann, Birger von Tiedemann, Andreas Pathogens Article In the last decade, the cultivated area of maize has increased in Central Europe due to its high yield potential and diverse uses for feed and bio-energy. This has led to more intense maize cultivation, with narrowed crop rotations resulting in the increase in maize leaf diseases. During 2012 and 2013, an inventory of maize leaf spot diseases was carried out in various regions in Central Europe. In addition to the major leaf pathogens, isolates of Phoma-like species were obtained from oval to elliptical spots on leaves or found in lesions produced by other leaf pathogens. A total of 16 representative Phoma-like strains were characterised for their pathogenicity on maize leaves, for their morphological characteristics and with a phylogenetic analysis based on multilocus sequence analysis using part of the actin (ACT), calmodulin (CAL), β-tubulin (TUB), internal transcribed spacer (ITS) region of ribosomal DNA and large subunit ribosomal RNA (LSU) genes. The strains were grouped into four clades, and morphological studies supported this classification for most of them. Strains were compared with six reference Phoma-like species strains from the Westerndijk Fungal Biodiversity Institute collection reported to colonise maize. The pathogenic group of strains from our collection (after completion of Koch’s postulates) did not cluster with any of these species, indicating a different and novel Phoma-like species infecting maize leaves. To our knowledge, this is the first study dissecting the Phoma species complex on maize leaves in Central Europe. MDPI 2021-09-18 /pmc/articles/PMC8467443/ /pubmed/34578248 http://dx.doi.org/10.3390/pathogens10091216 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramos Romero, Lucia
Tacke, Dagmar
Koopmann, Birger
von Tiedemann, Andreas
First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe
title First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe
title_full First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe
title_fullStr First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe
title_full_unstemmed First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe
title_short First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe
title_sort first characterisation of the phoma species complex on maize leaves in central europe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467443/
https://www.ncbi.nlm.nih.gov/pubmed/34578248
http://dx.doi.org/10.3390/pathogens10091216
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