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Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation

Tissue engineering offers auspicious opportunities in oral and maxillofacial surgery to heal bone defects. For this purpose, the combination of cells with stability-providing scaffolds is required. Jaw periosteal cells (JPCs) are well suited for regenerative therapies, as they are easily accessible...

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Autores principales: Weber, Marbod, Umrath, Felix, Steinle, Heidrun, Schmitt, Lukas-Frank, Yu, Lin Tzu, Schlensak, Christian, Wendel, Hans-Peter, Reinert, Siegmar, Alexander, Dorothea, Avci-Adali, Meltem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467579/
https://www.ncbi.nlm.nih.gov/pubmed/34576103
http://dx.doi.org/10.3390/ijms22189942
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author Weber, Marbod
Umrath, Felix
Steinle, Heidrun
Schmitt, Lukas-Frank
Yu, Lin Tzu
Schlensak, Christian
Wendel, Hans-Peter
Reinert, Siegmar
Alexander, Dorothea
Avci-Adali, Meltem
author_facet Weber, Marbod
Umrath, Felix
Steinle, Heidrun
Schmitt, Lukas-Frank
Yu, Lin Tzu
Schlensak, Christian
Wendel, Hans-Peter
Reinert, Siegmar
Alexander, Dorothea
Avci-Adali, Meltem
author_sort Weber, Marbod
collection PubMed
description Tissue engineering offers auspicious opportunities in oral and maxillofacial surgery to heal bone defects. For this purpose, the combination of cells with stability-providing scaffolds is required. Jaw periosteal cells (JPCs) are well suited for regenerative therapies, as they are easily accessible and show strong osteogenic potential. In this study, we analyzed the influence of uncoated and polylactic-co-glycolic acid (PLGA)-coated β-tricalcium phosphate (β-TCP) scaffolds on JPC colonization and subsequent osteogenic differentiation. Furthermore, interaction with the human blood was investigated. This study demonstrated that PLGA-coated and uncoated β-TCP scaffolds can be colonized with JPCs and further differentiated into osteogenic cells. On day 15, after cell seeding, JPCs with and without osteogenic differentiation were incubated with fresh human whole blood under dynamic conditions. The activation of coagulation, complement system, inflammation, and blood cells were analyzed using ELISA and scanning electron microscopy (SEM). JPC-seeded scaffolds showed a dense cell layer and osteogenic differentiation capacity on both PLGA-coated and uncoated β-TCP scaffolds. SEM analyses showed no relevant blood cell attachment and ELISA results revealed no significant increase in most of the analyzed cell activation markers (β-thromboglobulin, Sc5B-9, polymorphonuclear (PMN)-elastase). However, a notable increase in thrombin-antithrombin III (TAT) complex levels, as well as fibrin fiber accumulation on JPC-seeded β-TCP scaffolds, was detected compared to the scaffolds without JPCs. Thus, this study demonstrated that besides the scaffold material the cells colonizing the scaffolds can also influence hemostasis, which can influence the regeneration of bone tissue.
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spelling pubmed-84675792021-09-27 Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation Weber, Marbod Umrath, Felix Steinle, Heidrun Schmitt, Lukas-Frank Yu, Lin Tzu Schlensak, Christian Wendel, Hans-Peter Reinert, Siegmar Alexander, Dorothea Avci-Adali, Meltem Int J Mol Sci Article Tissue engineering offers auspicious opportunities in oral and maxillofacial surgery to heal bone defects. For this purpose, the combination of cells with stability-providing scaffolds is required. Jaw periosteal cells (JPCs) are well suited for regenerative therapies, as they are easily accessible and show strong osteogenic potential. In this study, we analyzed the influence of uncoated and polylactic-co-glycolic acid (PLGA)-coated β-tricalcium phosphate (β-TCP) scaffolds on JPC colonization and subsequent osteogenic differentiation. Furthermore, interaction with the human blood was investigated. This study demonstrated that PLGA-coated and uncoated β-TCP scaffolds can be colonized with JPCs and further differentiated into osteogenic cells. On day 15, after cell seeding, JPCs with and without osteogenic differentiation were incubated with fresh human whole blood under dynamic conditions. The activation of coagulation, complement system, inflammation, and blood cells were analyzed using ELISA and scanning electron microscopy (SEM). JPC-seeded scaffolds showed a dense cell layer and osteogenic differentiation capacity on both PLGA-coated and uncoated β-TCP scaffolds. SEM analyses showed no relevant blood cell attachment and ELISA results revealed no significant increase in most of the analyzed cell activation markers (β-thromboglobulin, Sc5B-9, polymorphonuclear (PMN)-elastase). However, a notable increase in thrombin-antithrombin III (TAT) complex levels, as well as fibrin fiber accumulation on JPC-seeded β-TCP scaffolds, was detected compared to the scaffolds without JPCs. Thus, this study demonstrated that besides the scaffold material the cells colonizing the scaffolds can also influence hemostasis, which can influence the regeneration of bone tissue. MDPI 2021-09-14 /pmc/articles/PMC8467579/ /pubmed/34576103 http://dx.doi.org/10.3390/ijms22189942 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weber, Marbod
Umrath, Felix
Steinle, Heidrun
Schmitt, Lukas-Frank
Yu, Lin Tzu
Schlensak, Christian
Wendel, Hans-Peter
Reinert, Siegmar
Alexander, Dorothea
Avci-Adali, Meltem
Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation
title Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation
title_full Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation
title_fullStr Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation
title_full_unstemmed Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation
title_short Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation
title_sort influence of human jaw periosteal cells seeded β-tricalcium phosphate scaffolds on blood coagulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467579/
https://www.ncbi.nlm.nih.gov/pubmed/34576103
http://dx.doi.org/10.3390/ijms22189942
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