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Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring

A fully automated sequential injection system was tested in terms of its application in liberation testing, and capabilities and limitations were discussed for clotrimazole liberation from three semisolid formulations. An evaluation based on kinetic profiles obtained in short and longer sampling int...

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Autores principales: Sklenářová, Hana, Beran, Marek, Novosvětská, Lucie, Šmejkalová, Daniela, Solich, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467677/
https://www.ncbi.nlm.nih.gov/pubmed/34577009
http://dx.doi.org/10.3390/molecules26185538
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author Sklenářová, Hana
Beran, Marek
Novosvětská, Lucie
Šmejkalová, Daniela
Solich, Petr
author_facet Sklenářová, Hana
Beran, Marek
Novosvětská, Lucie
Šmejkalová, Daniela
Solich, Petr
author_sort Sklenářová, Hana
collection PubMed
description A fully automated sequential injection system was tested in terms of its application in liberation testing, and capabilities and limitations were discussed for clotrimazole liberation from three semisolid formulations. An evaluation based on kinetic profiles obtained in short and longer sampling intervals and steady-state flux values were applied as traditional methods. The obtained clotrimazole liberation profile was faster in the case of Delcore and slower for Clotrimazol AL and Canesten cream commercial formulations. The steady-state flux values for the tested formulations were 52 µg cm(−2) h(−1) for Canesten, 35 µg cm(−2) h(−1) for Clotrimazol AL, and 7.2 µg cm(−2) h(−1) for Delcore measured in 4 min sampling intervals. A simplified approach for the evaluation of the initial rate based on the gradient between the second and third sampling points was used for the first time and was found to correspond well with the results of the conventional methods. A comparison based on the ratio of the steady-state flux and the initial rate values for Canesten and Clotrimazol AL proved the similarity of the obtained results. The proposed alternative was successfully implemented for the comparison of short-term kinetic profiles. Consequently, a faster and simpler approach for dissolution/liberation testing can be used.
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spelling pubmed-84676772021-09-27 Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring Sklenářová, Hana Beran, Marek Novosvětská, Lucie Šmejkalová, Daniela Solich, Petr Molecules Article A fully automated sequential injection system was tested in terms of its application in liberation testing, and capabilities and limitations were discussed for clotrimazole liberation from three semisolid formulations. An evaluation based on kinetic profiles obtained in short and longer sampling intervals and steady-state flux values were applied as traditional methods. The obtained clotrimazole liberation profile was faster in the case of Delcore and slower for Clotrimazol AL and Canesten cream commercial formulations. The steady-state flux values for the tested formulations were 52 µg cm(−2) h(−1) for Canesten, 35 µg cm(−2) h(−1) for Clotrimazol AL, and 7.2 µg cm(−2) h(−1) for Delcore measured in 4 min sampling intervals. A simplified approach for the evaluation of the initial rate based on the gradient between the second and third sampling points was used for the first time and was found to correspond well with the results of the conventional methods. A comparison based on the ratio of the steady-state flux and the initial rate values for Canesten and Clotrimazol AL proved the similarity of the obtained results. The proposed alternative was successfully implemented for the comparison of short-term kinetic profiles. Consequently, a faster and simpler approach for dissolution/liberation testing can be used. MDPI 2021-09-12 /pmc/articles/PMC8467677/ /pubmed/34577009 http://dx.doi.org/10.3390/molecules26185538 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sklenářová, Hana
Beran, Marek
Novosvětská, Lucie
Šmejkalová, Daniela
Solich, Petr
Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring
title Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring
title_full Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring
title_fullStr Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring
title_full_unstemmed Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring
title_short Sequential Injection Analysis for Automation and Evaluation of Drug Liberation Profiles: Clotrimazole Liberation Monitoring
title_sort sequential injection analysis for automation and evaluation of drug liberation profiles: clotrimazole liberation monitoring
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467677/
https://www.ncbi.nlm.nih.gov/pubmed/34577009
http://dx.doi.org/10.3390/molecules26185538
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