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Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice
A considerable effort has been devoted in all countries to react to the COVID-19 pandemic by tracing infected individuals, containing the spread of the disease, identifying therapies, and producing and distributing vaccines. Currently, a significant concern is the appearance of variants of the virus...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467753/ https://www.ncbi.nlm.nih.gov/pubmed/34573410 http://dx.doi.org/10.3390/genes12091428 |
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author | Anaclerio, Federico Ferrante, Rossella Mandatori, Domitilla Antonucci, Ivana Capanna, Matteo Damiani, Verena Tomo, Pamela Di Ferrante, Roberto Ranaudo, Marianna De Laurenzi, Vincenzo Stuppia, Liborio De Fabritiis, Simone |
author_facet | Anaclerio, Federico Ferrante, Rossella Mandatori, Domitilla Antonucci, Ivana Capanna, Matteo Damiani, Verena Tomo, Pamela Di Ferrante, Roberto Ranaudo, Marianna De Laurenzi, Vincenzo Stuppia, Liborio De Fabritiis, Simone |
author_sort | Anaclerio, Federico |
collection | PubMed |
description | A considerable effort has been devoted in all countries to react to the COVID-19 pandemic by tracing infected individuals, containing the spread of the disease, identifying therapies, and producing and distributing vaccines. Currently, a significant concern is the appearance of variants of the virus that may frustrate these efforts by showing increased transmissibility, increased disease severity, reduced response to therapy or vaccines, and ability to escape diagnosis. All countries have therefore devoted a massive attempt to the identification and tracking of these variants, which requires a vast technological effort to sequence a large number of viral genomes. In this paper, we report our experience as one of the Italian laboratories involved in SARS-CoV-2 variant tracing. We summarize the different approaches used, and outline a potential model combining several techniques to increase tracing ability while at the same time minimizing costs. |
format | Online Article Text |
id | pubmed-8467753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84677532021-09-27 Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice Anaclerio, Federico Ferrante, Rossella Mandatori, Domitilla Antonucci, Ivana Capanna, Matteo Damiani, Verena Tomo, Pamela Di Ferrante, Roberto Ranaudo, Marianna De Laurenzi, Vincenzo Stuppia, Liborio De Fabritiis, Simone Genes (Basel) Article A considerable effort has been devoted in all countries to react to the COVID-19 pandemic by tracing infected individuals, containing the spread of the disease, identifying therapies, and producing and distributing vaccines. Currently, a significant concern is the appearance of variants of the virus that may frustrate these efforts by showing increased transmissibility, increased disease severity, reduced response to therapy or vaccines, and ability to escape diagnosis. All countries have therefore devoted a massive attempt to the identification and tracking of these variants, which requires a vast technological effort to sequence a large number of viral genomes. In this paper, we report our experience as one of the Italian laboratories involved in SARS-CoV-2 variant tracing. We summarize the different approaches used, and outline a potential model combining several techniques to increase tracing ability while at the same time minimizing costs. MDPI 2021-09-16 /pmc/articles/PMC8467753/ /pubmed/34573410 http://dx.doi.org/10.3390/genes12091428 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Anaclerio, Federico Ferrante, Rossella Mandatori, Domitilla Antonucci, Ivana Capanna, Matteo Damiani, Verena Tomo, Pamela Di Ferrante, Roberto Ranaudo, Marianna De Laurenzi, Vincenzo Stuppia, Liborio De Fabritiis, Simone Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice |
title | Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice |
title_full | Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice |
title_fullStr | Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice |
title_full_unstemmed | Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice |
title_short | Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice |
title_sort | different strategies for the identification of sars-cov-2 variants in the laboratory practice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467753/ https://www.ncbi.nlm.nih.gov/pubmed/34573410 http://dx.doi.org/10.3390/genes12091428 |
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